J.EXPERIMENTAL & CLINICAL MEDICINE
2018, ¹ 6
1. A.ARCHVADZE, A.KISTAURI, N.GONGADZE, T.MAKHARADZE, M.GOGOLAURI, K.CHIRAKADZE
MODERN TREATMENT APPROACHES IN THE MANAGEMENT OF DIABETES MELLITUS
Tbilisi State Medical University, Department of Internal Medicine, University Hospital, Department of Medical Pharmacology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-8-17
According to the World Health Organization, the number of people with diabetes mellitus increases yearly; namely, in 2014 more than 422 million people were suffered from diabetes, while in 1980 the number was 108 million. From top ten countries with high prevalence of diseases dominate China, the United States, Italy, Russia, Japan, and India.
Diabetes mellitus is a chronic disease proceeding with hyperglycemia. The elevated blood glucose level activates of a number of various mechanisms which can injure the cardiovascular system, eyes, kidneys and neurons.
The main goal of diabetes treatment is to reduce the severity of disease and prevent the formation of various organ complications. Consequently, the strategy of antidiabetictherapy is tomaintain the normal blood glucoselevel by insulin administration (in case of insulin production deficiency) and by other anti-diabetic drugs (in case of insulin receptor resistance). Majority of the antdiabetic drugsprevents microvascular complications and does not protect patients from macrovascular injuries.
Currently, other targets that are involved in the development of diabetes are also considered. In this regard, it is remarkable to use vaccines, which reduces autoimmune processes and respectively protects the pancreatic beta cells from the autonomous antibodies produced against pancreatic autoantigens.
Despite of advances in the diabetes mellitus treatment strategy, due to the frequency of microvascular and macrovascular injuries arises in the diabetic patients; the management of diabetes mellitus is still remainedas an unsolved problem of complex approach.
2. N.SULASHVILI, M.BEGLARYAN
DRUGS DISPENSERS’ PROFESSIONAL CHOICE AND INFLUENCING FACTORS ON PHARMACEUTICAL ACTIVITIES IN GEORGIA
Yerevan State Medical University, Department of Pharmaceutical Management, Armenia
J. Experimental and Clinical Medicine, 2018, 6, pp-17-22
Introduction. Psychological studies show that the influence of these factors on the vocational choice is different. It was established that a sufficiently high level of education of the mother’s or the father’s vocational status promotes a deal of the children . The parents’ mind about the occupational choice and the influence of the parents is stronger than the influence of teachers and specialists in career counseling, and at the same time, the parents’ opinion could act as a factor of inhibition in the occupational development of an individual in the period of professional finding [2,3,4]. The given scientific research enables us to identify various approaches to the choice of structural components of the process of professional development in phases, stages, levels and directions. At the same time the professional formation of specialists is observed as the accumulation of professional knowledge, skills and experience from the beginning of the formation of professional intentions until the end of effective professional activity [5,6]. Studying of the accessible literature enabled us to determine the factors influencing on the professional choice of a person: family position, a position of the school pedagogical collective (mentor and a class teacher), the person’s professional and life plans, abilities and manifestations, inclinations, the claim for public recognition, awareness of a particular professional activity [7,8,9].
GOALS. Aim and objects was to study and analyze drugs dispensers’ professional choice and influencing factors on pharmaceutical activities in Georgia.
MATERIALS AND METHODS. Research objects are materials of sociological research: The study was quantitative investigation and analysis of drugs dispensers’ professional choice and influencing factors on pharmaceutical activities in Georgia by using survey (Questionnaire). Surveys was for pharmacists, 810 pharmacists were interviewed in Georgia. The survey was conducted on 09.09.2016 - 30.04.2017. We used methods of systematic, sociological (surveying, questioning), comparative, mathematical-statistical, graphical analysis. The data was processed and analyzed with the SPSS program.
RESULTS AND DISCUSSIONS. On the basis of performed study results the following have been founded.
3. L.LURSMANASHVILI, G.KVARATSKHELIA, M.MACHAVARIANI, M.ENUKIDZE, L.GULUA, N.KIPIANI, N.LOBZHANIDZE, T.SANIKIDZE
INDIVIDUAL AND COMPLEX ANTIOXIDANT ACTIVITY OF VITAMINS AND EXTRACTS OF GREEN TEA
Georgian David Aghmashenebeli University; Tbilisi State Medical University V.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-22-26
The aim of the research was to study the antioxidant activity of green tea extracts on the experimental model of Jurkat cells.
Based on the analysis of the results of the survey, individual and complex activity of various vitamins (C, E) and herbal extracts (green tea, green tea catechins, green tea pectin) have been identified, which should be considered during their medicinal and prophylactic use.
4.1J.CHEISHVILI, 2I.ZARNADZE, 2SH.ZARNADZE, 1A.KURTSIKIDZE, 1N.TSERETELI
STUDY OF POTENTIAL MIGRATION BEHAVIOUR AMONG GEORGIAN STUDENTS
1Akaki Tsereteli State University, Kutaisi; 2Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-26-28
The social-economical and political changes of the last decade has influenced the mentality of new generation through the country. Especially the attitude toward potential migration level in the students from higher education institutions. An increasing level of unemployment alongside a decreasing income and decreasing potential of the educational system are all a driving forse for Georgian students to study/live/ work abroad.
THE EFFECT OF THE DRUG RIDUTOX ON THE INTENSITY OF LIPID PEROXIDATION AND ITS ROLE IN RESTORING THE ANTITOXIC FUNCTION OF LIVER
Azerbaijan State Medical University, Baku
J. Experimental and Clinical Medicine, 2018, 6, pp-28-34
The aim of the work was to study the effect of the drug Ridutox, after its introduction into the abdominal cavity, on the intensity of lipid peroxidation that occurs in the liver tissue.
The studies were conducted at the Research Center of the Azerbaijan Medical University on 40 rabbits of the chinchilla breed. To determine the concentration of diene conjugates and hydrogen peroxide, which are considered the initial product of lipid peroxidation, the method I.D. Stalnoy. Statistical data processing included the calculation of the arithmetic mean, its errors.
The results obtained during the experiments show that the exotoxicosis created by inhalation of HCl, affecting the liver, upsets the balance between lymph circulation and the microcirculatory bed, creating oxidative stress in hepatocytes, disrupting the metabolic process. Ridutox enhances the body’s antioxidant defense system. In the liver tissue, the concentration of H2O2 dynamically increases, there is a sharp change in the lipid peroxidation of MDA. The antioxidant activity of the liver tissue increases dramatically, the concentration of medium molecular peptides decreases. Creatinine in the blood plasma is slightly higher compared with the norm. The balance between lymph circulation and the microvasculature is disturbed.
6. T.MIKELADZE, L.ZHORZHOLIANI, L.SAGINADZE, G.ARVELADZE, I.SULABERIDZE
DIFFERENTIAL CHARACTERISTICS OF PREDICTIVE INDEX OF PREVENTION AND INCIDENCE OF ASTMA (PIAMA) IN YOUNG CHILDREN WITH RECURRENT WHEEZING
Davit Aghmashenebeli University of Georgia (DAUG), Evex Regional Hospital;
Curatio Clinic, Institute of Pediatrics - Allergy Center, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-34-41
The aim of the present study was to investigate differential characteristics and predictive value of PIAMA index in children with recurrent wheezing of different phenotypes.
120 patients, 5 to 7 years of ages, with wheezing syndromes, have been involved in the study. According to the study of PIAMA index prognostic variables, bronchial asthma was diagnosed in 18.8% of patients with multi-factorial wheezing
The relative chance of developing a wheezing (OR-17.5695% CI 4.62-78.21) was high when: without response to a cold, parents taking inhaled medications (OR-2.18.95% CI 0.86-7.35), overdue fetus (OR-2.11, 95% CI 0.38-15.24) and physician-diagnosed eczema (OR-2.38, 95% CI 1.01-5.66).
The high prognostic value of wheezing developed: in case of overduebaby (80%), without a cold (94%) and/or taking inhaled medications by parents (79%) was observed in children with multifactorial wheezing.
PIAMA index gives makes it possible to identify and differentiate the children with a high and/or low risk of bronchial asthma formation, in terms of reducing the use of hormone preparations and providing adequate treatment.
Determining the level of asthma predictive index (API) and NO at early ages is of great importance to identify the contingent with high probability/predictive risk of bronchial asthma formation, especially in young children affected with multifactorial wheezing.
7. R.VEKUA, A.ZEDGINIDZE, G.ORMOTSADZE
THE MICKONUCLEUS TEST AS ENVIRONMENTAL FACTORS IN IMPACT ASSESSMENT CYTOGENETICAL METHOD
I.Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-41-43
Were studied influencing of Chiatura Manganese enrichment enterprise on the health condition of inhabitants in villages Khreiti, Perevisa and Rgani. The micronucleus test has identified the micronuclei and other nuclear protrusions quantitative changes in oral cavity exfoliative buccal cells.
8. T.MAMINAISHVILI, M.ENUKIDZE, M.MACHAVARIANI,
M.PAPAVA, I.CHKHIKVISHVILI, NINA KIPIANI, T.SANIKIDZE
NO CONTENT IN JURKAT AND MDCK CELLS INCUBATED IN OXIDATIVE STRESS CONDITIONS UNDER THE INFLUENCE OF GEORGIAN LEGUMINOUS CROPS CULTURES
Tbilisi State Medical University; V.Bakhutashvili Institute of Medical Biotechnology;
David Aghmashenebeli University of Georgia, Tbilisi
J. Experimental and Clinical Medicine, 2018, 6, pp-43-48
The identification and investigation of natural compounds that impart anti-inflammatory, antioxidant activity will enable their use with the purpose of preventing various chronic diseases. The elucidation of new mechanisms and signaling pathways involving NO is related to ability to specifically, selectively and sensitively detect and quantify NO and all relevant NO products and metabolites in complex biological matrices.
The aim of the study was to identify the impact of Georgia leguminous crops cultures on the nitric oxide (NO) content inJurkat and MDCK cells incubated at the oxidative stress conditions.
Research results revealed that Georgian leguminous crops didn’t influenced on the NO content in the intact Jurkat and MDCK cells; more effective in the process of the normalization of NO content in immune and epithelial cells cultures incubated under oxidative stress conditions were Batumian beans and beans “Kidney” extracts.
9. B.KORSANTIA, S.KUPRADZE, M.AKHVLEDIANI, V.MALATSIDZE, I.KHARISCHARISHVILI, N.BORCHKHADZE
ASSESSMENT OF MECHANISMS OF PROTECTIVE PROPERTIES OF THE ZEOLITE DIET IN CERTAIN CLINICAL AND EXPERIMENTAL PATHOLOGIES OF THE GASTROINTESTINAL TRACT
Tbilisi State Medical University, Dept of Inner Medicine and V.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-48-55
The purpose of our work was to study pharmacodynamics of the clinoptilolite and his immunotropic properties, also estimation clinical condition of patients with some diseases of the gastrointestinal tract on the background of the zeolite “diet”. In experiments on white mice, cytomorphological features of the tissues of the small intestine and some organs of the abdominal cavity in aflatoxicosis were also studied.
The preparation possesses a strong protective action on organism of patients: observed weakening of dyspeptic effects, the termination of asthenic-vegetative syndrome, reducing the size and normalization of the liver function test, rapid relief of intoxications, decrease in terms of hospitalisation of patients. Zeolite “diet” has been followed by the improvement of functional indices of blood and normalization of the immunological parameters, especially interferon system.
Natural zeolites have the ability to bind toxins, neutralize and destroy them, excrete from organism. Zeolites promoted strengthening of phagocitosis, increased quantity of phagosomes and lisosomes, i.e. raised functional activity of cells. Zeolite diet contributed to the activation of phagocytosis in Kupffer cells of the liver and antioxidant protection in intact white mice. Acute aflatoxicosis induced mortality of white mice in 75% of cases. Zeolite diet reduced the rate to 40% and normalized parameters of phagocytosis and hypoxia.
The results of experiments showed that the particles of clinoptilolite (in the forage) are able to induce a more active immune-morphogenesis in intestinal tissues, liver, thymus, regional lymph nodes, spleen of white mice.
10. G.GURGENIDZE, D.LABARTKAVA
EFFECTIVENESS OF IMMUNOTHERAPY WITH PERORAL BACTERIAL LYSATE IN PATIENTS WITH DIFFERENT CLINICAL FORMS OF BRONCHIAL ASTHMA
V.Bakhutashvili Institute of Medical Biotechnology of Tbilisi State Medical University,
J. Experimental and Clinical Medicine, 2018, 6, pp-55-59
The effectiveness of oral immunotherapy with polyvalent lysate of bacterial origin (Bronchomunal LEK), consisting from antigens derived via mechanical lysis of eight different bacterial strains of upper respiratory system (Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Klebsiella ozaenae, Staphylococcus aureus, Streptococcus viridans, Streptococcus pyogenes, Moraxella catarrhalis) for the treatment of asthmatic patients with different sensitivity to the anti-inflammatory action of corticosteroids (steroid-sensitive and steroid-resistant clinical forms of asthma). Peroral immunization of patients with polyvalent lysate after 3 monts resulted in significant improvement of their symptoms, reduction of oral and inhaled steroid dosage, decrease in frequency of exacerbations, requiring patient hospitalization and marked improvement of spirometric values. After ending of treatment these effects were retained at least for several months.
11. I.KHARISCHARISHVILI, B. KORSANTIA
METHODS FOR THE CORRECTION OF ANTHROPOGENIC POLLUTION BY BIOGENIC STIMULANTS
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-59-61
Increased anthropogenic pressure on nature and environmental pollution is the most serious problem for modern science. Harmful substances cause different pathologies and chronic diseases. The struggle against them requires new effective and safe means for treatment and prophylactic. Immunodepression and chronic effects of different pollutants are a frequent problem of the inhabitants of the megapolis. This issue is no exception and in 2012, in a study of the WHO, our country came in 2nd place in terms of air pollution.
In solving this problem, nutrient stimulators of natural origin can provide a great help as a promising direction for the treatment of chronic conditions caused by pollution.
12. N.JANKARASHVILI, S.KAKHADZE, M.TOPESHASHVILI
NEOADJUVANT VMAT RADIOCHEMOTHERAPY WITH A SIB COMPARED TO STANDARD CHEMORADIATION FOR LARC
Radiation Oncology Department of Academician F.Todua Medical Center-Research Institute of Clinical Medicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 6, pp-61-66
Locally advanced rectal cancer includes cancers which are extending through the rectal wall, or involving locoregional lymph nodes. Standard management for locally advanced rectal cancer (LARC; stage II or III) consists of preoperative (rather than postoperative) chemoradiotherapy (CRT), radical transabdominal surgery, and postoperative chemotherapy.
There are several clinical trials of preoperative CRT for LARC using SIB-VMAT, reporting good oncologic outcomes with same toxicities. Based on these clinical data, we commenced SIB-VMAT for PCRT in LARC.