J.EXPERIMENTAL & CLINICAL MEDICINE
2018, ¹ 5
1. M.TSIMAKURIDZE, E.MAISURADZE, D.ZURASHVILI, M.MATHESHVILI,
MAIA TSIMAKURIDZE, N.KHACHAPURIDZE
SOME BIOCHEMICAL FINDINGS OF VIBRATION DISEASE
TSSU, Department of Environmental Health and Occupational Medicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-16-18
Early identification of health effects is the priority of modern occupational medicine. It will make them express during the secondary prevention and effective control of work-related pathologies. The purpose of investigation was to identify some changes in biochemical findings of hepopexis and anticoagulation systems. The results of investigation shows changes of hepopexis and anticoagulation systems and derangement of liver, which moderately depends on the degree of pathological process.
2. 1D.TOPHURIA, 2M.MATOSHVILI, 3L.BENASHVILI, 4N.SULASHVILI
LIVER TOXIC DAMAGE IN OCCUPATIONAL EXPOSURE TO SOLVENTS
Tbilisi State Medical University, Departments: 1Human Normal Anatomy, 2Dermatology and Venereology, 3Topographic Anatomy and Operative Surgery, 4University of Georgia, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-18-20
Liver is the main organ responsible for the metabolism of drugs and toxic chemicals, and so is the primary target organ for many organic solvents. Organic solvents used in different industrial processes may be associated with hepatotoxicity. Several factors contribute to liver toxicity; among these are: species differences, nutritional condition, genetic factors, interaction with medications in use, alcohol abuse and interaction, and age. The main pathogenic mechanisms responsible for functional and organic damage caused by solvents are: inflammation, dysfunction of cytochrome P450, mitochondrial dysfunction and oxidative stress.
3. A.MAKALATIA, E.MIRVELASHVILI
ORAL HEALTH KNOWLEDGE ASSESSMENT IN KVEMO KARTLI REGION
TSMU, Odontology Department; Department of Public health management,
Policy and Economics, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-20-22
Oral cavity health is significant determinant for general health and represents the concern of the public health bodies. The goal of our research was the assessment of the knowledge level on the oral cavity health in different groups of patients living in regions of Georgia.
The research results are as follows: The level of knowledge concerning the oral cavity health issues is low and in general variation, is less than the standard average index. The opinion is just for the oral cavity hygiene as well as the level of knowledge on oral cavity diseases. In different patient groups the knowledge level is different, statistically significant difference was found between the employed and unemployed persons, women and men. As a result of our research it was found also, that the knowledge level on the oral cavity hygiene and the oral cavity diseases was comparatively high in women samples and the employed patients group.
4. N.S.VACHNADZE, T.SH.SULADZE, V.Y.VACHNADZE
PHYTOCHEMICAL SCREENING OF PLANTS OF THE GENUS MAGNOLIA, INTRODUCED INTO WESTERN GEORGIA
Tbilisi State Medical University, Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-22-25
15 species of Magnolia were subjected to phytochemical screening for their content of alkaloids and such natural organic compounds as flavonoids, tanninsand phenolic compounds. On the basis of the data obtained, it was established that the aerial plant organs contain moderate amountsof flavonoids and tannins, in addition to alkaloids, while phenolic compounds are present in trace amounts. Among the alkaloids present in the vegetative organs, aporfin bases are dominant, namely: lyriodenin, lanuginosin, caaverin, remmerin andmagnoflorin, aquaternary base. For further research, the following species deserve attention: Magnolia acuminata L., Magnolia delavayi Franch., Magnolia fuscata Andrz.
5. 1D.TOPHURIA, 2M.MATOSHVILI, 3L.BENASHVILI, 4I.KOKHREIDZE, 4B.SAMKHARADZE
DRUG-MEDIATED LIVER INJURY IN OBESITY
Tbilisi State Medical University Departments: 1Human Normal Anatomy1, 2Dermatology and Venereology2, 3Topographic Anatomy and Operative Surgery3, 4Oncology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-25-28
World many countries are facing an epidemic of obesity that can be explained, at least in part, by a sedentary life style and calorie overconsumption. This poses a major issue for public health since obesity primarily enhances the risk of various illnesses such as type 2 diabetes, coronary heart disease, some cancers and non-alcoholic fatty liver disease (NAFLD). Consequently, obese patients are consuming on average more drugs than non-obese individuals, some medications in obese individuals can cause severe liver damage.
6. D.TURABELIDZE, B.KIKALISHVILI, N.GORGASLIDZE,
TS.SULAKVELIDZE, M.MALANIA
THE STUDY OF LIPIDS OF SOME PLANTS CULTIVATED IN GEORGIA
TSMU I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-28-30
From the seeds of Vitex agnus castus L. and Cucurbita pepo duch. cultivated on the experimental field of medicinal plants of the I.Kutateladze Pharmacochemistry Institute there were isolated sums of neutral and pollar lipids. There were revealed the classes in them. With the help of high performance liquid chromatography (HPLC) there were identified saturated, unsaturated and polyunsaturated fatty acids. In the pollar sum of the seeds Vitex agnus castus L. there were revealed four, in the pollar sum of the seeds Cucurbita maxima duch. six, but in the pulp of Cucurbita maxima duch. five phospholipids. By the pharmacologically study there was established that the oil of the seed Cucurbita maxima duch. possess the gastroprotective action.
7. N.NEMSITSVERIDZE, T.CHUMBURIDZE, T.ZARKUA, I.TSURTSUMIA,
N.NIKURADZE, N.DUGASHVILI
ASPECTS OF PHARMACEUTICAL SURVEILLANCE IN HEALTHCARE
TSMU Department of Social and Clinical Pharmacy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-30-31
As it has been noted, recently, the focus from the traditional drug preparation at the chemist’s was moved to the issues related to the patient health care. Consequently, the main function of pharmacist such as manufacturing and releasing the pharmaceutical products, has been changed and nowadays, modern pharmacist appeared to be provider of patient safety surveillance. It was quite an affair to observe how pharmaceutical care is carried out at the pharmacies in Tbilisi. The study was conducted by using the questionnaire-survey. 30 pharmacists and 30 patients of the pharmaceutical companies “Aversi” “PSP” and “GPC” were surveyed. Based on the analysis of the obtained data can be assumed that pharmaceutical surveillance is not carried out sufficiently, as all pharmacists working in the pharmacies are not sufficiently competent in main aspect of pharmaceutical surveillance – clinical pharmacy. Therefore, increase the knowledge of pharmacists in clinical pharmacy and pharmacotherapy, which is possible not only by trainings, but primarily, through the development of pharmaceutical surveillance in Bachelor programme and implementation of Master programme in clinical pharmacy have been recommended.
8. N.GORGASLIDZE, L.NADIRASHVILI, G.ERKOMAISHVILI
METROLOGICAL CHARACTERISTICS OF THE METHOD FOR DETERMINING THE ACTIVITY OF FICIN - A PROTEOLYTIC ENZYME IN THE LATEX OF IMMATURE FRUITS OF FIG TREES (FICUS CARICA)
Tbilisi State Medical University, Iovel Kutateladze Institute of Pharmacochemistry,
Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-31-34
The method of obtaining dry milky juice from the petioles of immature fruits of fig trees, the yield of the dry latex, as well as the sensitivity, reproducibility and accuracy of the method for determining the activity of ficin, a proteolytic enzyme that it contains have been studied.
The obtained data show, that the method is reproducible, simple and accurate. The relative error of the average results (ε) is in the range of 2-4%. The results of the study can be used to determine the proteolytic activity of ficin, contained in the latex of immature fruits of fig trees and subsequently to analyse medicinal preparations containing this enzyme.
9. P.JAVICH, M.KAKHETELIDZE, L.CHURADZE, M.GABELAIA
ON THE ISSUE OF THE USE OF NATURAL RESOURCES OF GEORGIA FOR CREATING MEDICINAL AND COSMETIC MEANS
TSMU I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-34-36
Georgia is rich in a variety of natural resources that can be used for the production of a wide range of medicinal and cosmetic preparations. Prophylactic tooth pastes, ointments and elixirs were developed on the basis of mineral waters from “Lugela”, “Tskaltubo”, ”Amagleba” and “Sairme”.
The pseudovolcanic mud from Akhtala is used to treat a variety of diseases. Studies are being conducted for the creation of medicinal and cosmetic preparations, which will allow for the therapeutic muds to be used outside their place of origin.
10. A.TSIBADZE, N.TSKHVEDIANI, L.MIKELADZE, D.DELIBASHVILI, S.GOKADZE
THE EFFECT WHITE AND BLUE LIGHT LED BULB IRRADIATION ON HUMAN‘S
HAIR AND SKIN
Tbilisi State Medical Univeraity, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-36-38
We aimed to compare white Led light and in different factor and blue one as a comparative effect while irradiating the separate part of hair and hole organism as well. The used white and blue light Led bulb with the range of 480 nm frequency drop, causes changes in vegetable regulation mechanisms of the organism.
Ex vivo research, resulted photoinduced additional free radicals sharp short period raise while using blue red irradiation.
We may assume that photoinduced additional free radicals raise takes a leading role as we received no changes in organism‘s vegetable balance system while white led irradiation and blue led irradiation gave an effect of significant raise of stress index.
11. K.SHALASHVILI, T.SAGHAREISHVILI, M.ALANIA
PHENOLIC COMPOUNDS OF SOME PLANTS INTRODUCED IN GEORGIA
Tbilisi State Medical University, I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-38-41
The date on the composition of phenolic compounds of species introduced in Georgia - Ginkgo biloba L. (Ginkgoaceae), Cercis siliquastrum L. (Leguminosae), Fraxinus ornus L. (Oleaceae) are given in this paper. Flavonoid glycoside – myricitrin and oxycoumarin glycoside cichoriin were isolated and characterized from them.
The suitability of leaves of Ginkgo biloba growing in Georgia for producing vascular drug of „Ginkgo-Bath“ was established.
12. L.KINTSURASHVILI, K.MCHEDLIDZE, M.CHURADZE, V.MSHVILDADZE
MICROSTRUCTURAL CHARACTERISTICS OF THE NEEDLE OF TAXUS BACCATA L. (TAXACEAE), AS A CURATIVE HERBAL RAW MATERIAL
Tbilisi State Medical University, Iovel Kutateladze Institute of Pharmacochemistry,
Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-41-44
There were studied diagnostic microstructural characteristics of the needle of Taxus baccata L., spread in Georgia, as a curative herbal raw material. Abaxial and adaxial epidermis of rectilinear and right-angled based-cells of the needle are in a straight line. Medullated part of the needle has a dorsovetral structure and hypostomatic. Stomas apparatus of ventilatory system is complex, type of encyclocyte. Stomas in the needle’s epidermis is differentiated as rectilinear rows.
13. 1D.CHITASHVILI, 2E.KORINTELI, 2N.BERIANIDZE, 2A.GOBIRAKHASHVILI
The effect of geographic location and climatic conditions on the functional indices of cardio-respiratory system IN sportsmen
1Ilia State University; 2Georgian State Teaching University of Physical Education and Sport;
Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-44-47
The duration of adaptation of sportsmen of various qualifications was experimentally established in conditions of Bakuriani as compared with Tbilisi background indices taken in resting state and after the standard physical loading.
14. E. KORINTELI
Physical and Functional Data of Georgian Judo Youth (16-17 Years) Team in conditions of Passive and Active Rest usage
Georgian State Teaching University of Physical Education and Sport, Tbilisi
J. Experimental and Clinical Medicine, 2018, 5, pp-47-49
The better ways to use the passive and active rest (massage) in conditions of work with intervals are considered in this article. The above-mentioned has a great importance in definite types of sport (boxing, wrestling, and rowing).
15. K.KHAZARADZE, N.JAPARIDZE, V.SURGULADZE
THE IMPORTANCE OF ADAPTIVE SPORTS FOR PERSONS WITH DISABILITIES
Georgian State University of Physical Education and Sport, Tbilisi State Medical University,
Caucasian University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-49-51
The paper analyzes the situation regarding conditions of disabled persons in sport. The special attention is paid to disabled sportsman’s achievements. The issue concerns how expansion of paraport species can be done and ensure the involvement of young people in relevant species.
16. 1D.TOPHURIA, 2M.MATOSHVILI, 3L.BENASHVILI, 4I.KAKHNIASHVILI
Tbilisi State Medical University, Departments: 1Human Normal Anatomy1, 2Dermatology and Venereology, 3Topographic Anatomy and Operative Surgery3, 4Clinical Skills, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-51-53
Medication combinations that improve the efficacy of thiazolidinediones or ameliorate weight gain side effects of the therapy represent an attractive potential treatment for non- alcoholic steatohepatitis (NASH). The aim of this randomized, open label trial was to assess the efficacy of rosiglitazone and metformin in combination versus rosiglitazone and losartan, compared with rosiglitazone alone. The primary aim of this study was to assess for the differences between treatment groups in the improvement of steatosis, hepacellular inflammation, and fibrosis. Conclusion: Combination therapy with rosiglitazone and metformin or rosiglitazone and losartan confers no greater benefit than with rosiglitazone alone with respect to histopathology.
17. Z.KEMOKLIDZE, M.GETIA, N.GORGASLIDZE
ANALYSIS OF DYNAMICS OF CONCENTRATION OF THE ACTIVE SUBSTANCE FROM THE TABLETS OF FATSOFLOGIN FOR OPTIMIZATION OF PHARMACOTHERAPY
TSMU, I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-53-55
Were studied pharmacokinetic characteristics, which shows dependence of elimination process of the triterpene glycosides (Fatsiosid D), from the tablets of Fatsiflogin 0,05 in time. Experimental researches were provided in the solution of hydrochloric acid (pH 1,64) using the method of shaking. Was determined that, optimal elimination of the Fatsiosid D from the tablets of Fatsiflogin is occurred from the 45 to 90 minutes, maximal concentration of the triterpene glycoside is fixed around 1 hour.
18. 1T.G.GIORGADZE; 2S.G.GIORGADZE
PYROPTOSIS - POSSIBLE TARGET FOR ENVIRONMENTAL FACTORS
1School of Medicine of Grigol Robakidze University;
2TSMU, Department of Histology, Cytology and Embryology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-55-59
The research topic articles address the role of various environmental factors that may contribute to the onset of diseases. They are presented in the following order: purely environmental factors, dysbiotic conditions, and infections of bacterial, fungal, and viral nature. The newly pro-inflammatory form of programmed cell death was named pyroptosis and arises from microbial infection and danger signals, including crystalline substances, monosodium urate, asbestos, silica crystals toxins and extracellular ATP. In this review, we discussing the inductors, molecular mechanisms and morphological feature of pyroptosis. Pyroptosis is a form of inflammatory programmed cell death pathway activated by human and mouse caspase 1, human caspase 4 and caspase 5, or mouse caspase 11. Although Inflammatory programmed cell death is classified as canonical or non-canonical based on inflammatory caspases, the pore-forming effector protein, gasdermin-D (GSDMD) causes them to have identical morphological changes. By carefully examining the molecular processes that occur in dying cells, we will better characterize novel pathways of cell death and further our understanding of the pathologies underlying a variety of human health problems.
19. D.GABUNIA, A.KISTAURI
ATTENTION – POLYPRAGMAZIA
TSMU, Department of Internal Medicine of First University Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-59-61
The purpose of our work was to analyze the history of the hospital patients in 2016-2018 to study the issue of polyapragmassia. The history of 900 illnesses of patients treated in therapeutic, cardiological and neurological departments is considered. Age 40 to 88 years old. Women are 62%, men are 38%. The number of prescribed medicines varies from 7 to 22. One patient received 11 medicines daily on average. We should not mention more than 4 medicines (of course, in addition to urgent situations).Each new drug should be selected according to its instructions. Polypragmazia in financial point of view acts negatively for the stationary type medical institutions.
20. A.KARSANIDZE, N.ANTELAVA, N.GORGASLEDZE, M.GHONGHADZE, M.OKUDZHAVA, K.PACHKORIA
MARKETING RESEARCH OF STATINS IN THE PHARMACEUTICAL MARKET OF GEORGIA
Tbilisi State Medical University, Department of Pharmacotherapy; I.Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-61-65
It is established that 84 preparations from 22 countries, manufactured by 33 pharmaceutical companies were registered in Georgia, from the group of statins. The sources of information were analyzed, according to which respondents use statins. It is established that a large percentage of them use statins not for the doctor’s prescription, but according to information from advertising sources or the Internet. Statins are a highly active group of drugs, which, along with high efficiency, have side effects. Rational statin therapy may be carried out only on a doctor’s recommendation. Taking into account the risk factors for the development of side effects of statins in a particular patient, the correct choice of the drug, the dose and its dosage form can prevent the development of undesirable effects. The treatment of statins should be carried out under the close supervision of a physician. Statins from pharmacies should be given by prescription.
21. M.KAPANADZE
ASSESSMENT OF THE RISK OF POOR RESULT OF ENDOPROSTHETICS, FOR THE MODIFIED FLESH RELEASE METHOD IN TOTAL HIP JOINT ARTHROPLASTY ON THE BASIS OF DYSPLASTIC OSTEOARTHROSIS OF THIGH JOINT THAT DEVELOPED DUE TO ITS CONGENIAL DISLOCATION (CROWE III-IV)
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-65-68
The purpose of this retrospective study was to analyze the functional and radiographic results of total hip arthroplasty with modified flesh release method
Material and methods: We have studied the consequences of arthroplastic interventions among 106 patients, aged 18-85, who had Crowe III and IV types of dysplastic osteoarthrosis. Harris scale was used for the final assessment.
While planning the surgical procedure we use radiography and in case of necessity we apply to thigh joint CT with 3D reconstruction. Statistical analysis: According to the quantitative indicators, the credibility of the difference between the groups was determined by the student’s test for independent selection, and after the treatment and treatment - the student’s pair of tests. For Quality Amplification - Pirosone c2 criteria, the material was developed using SPSS 22 of the Statistical Programs Package.
Results: Using a modified method Hariis score increased for 40+19.0-93+8.1. Reduces the relative risk (RR=0.04, 95%CI:0.01-0.2) and attributable risk(ARR=0.24, 95%cCI: 0.15-0.33) of poor outcome, the number of patients who have to treat one positive result NNT=4,
Conclusion: modified flesh release method reduse risk of poor outcome in patient dysplastic osteoarthrosis Cruw III-IV grade.
22. L.BUGHADZE, L.ZHORZHOLIANI, N.MANJAVIDZE
VITAMIN A LEVEL AND COMORBID CONDITIONS OF CHILDREN WITH
BRONCHIAL ASTHMA
David Aghmashenebeli Georgian University, Tbilisi
J. Experimental and Clinical Medicine, 2018, 5, pp-68-73
The modern life style and lack of vitamin D through food makes vitamin deficiency even in the southern countries.
The study was performed in children aged 6-15 years, including patients with asthma (n=50), who referred Sachkhere Medical Center for visit. Diagnosis and control of asthma in the patients produces by the GINA guideline. The quantitative determination of vitamin D in blood serum (Chemiluminescentional analysis) was performed.
Individual study map was created for each patients, which included questions as a debut of the disease, symptoms and comorbid conditions. The genetically predisposition of the patients, allergic anamnesis and risk factors were also assessed.
Results. Using the multivariate logistic regression analysis, the presence of asthma was associated with reduced level of vitamin D (OR=1.35, 95% CI (1,14-1.58) P=0.011; X²=6.78; F-0.022). By the results Vitamin D deficiency is a reliable correlation with alimentary and medicine allergy in the first year of life (p-0.039 x2- 4.24), genetically predisposition of bronchial asthma (p-0.039; x2- 2.42) and atopic dermatitis (p-0.008; x2-2.32); but neither (p-0.08; x2-7.84) and urticaria (p-1.00; x2-10.9), nor (p-0.12; x2-5.12) was correlations with vitamin D level.
Conclusion. The presence of vitamin D deficiency effectively predict increased risk for childhood asthma; Serum vitamin D level is strongly associated with atopic dermatitis and genetically predisposition of asthma, but neither allergic rhinitis, nor pregnancy pathology, are correlated with vitamin D deficiency.
23. D.TABIDZE, L.BARAMIDZE, Z.SIKHARULIDZE, D.KITOVANI, M.KAJRISHVILI
STUDY OF SATISFACTION OF CLINICS AND PATIENTS INVOVLVING IN ELIMINATION PROGRAMME OF HEPATITIS C
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-73-76
Georgia belongs to the countries with high spread of hepatitis C. According to seroprevalence population research conducted by the National Centre for Disease Control and US Centres for Disease Control. In terms of heavy load of the disease, the Government of Georgia, actively supported by the international partners – US Centres for Disease Control and Prevention, pharmaceutical company “Gilead” and the WHO, set as a purpose elimination of hepatitis C epidemics in the country, in the issue of this Georgia became one of the first model countries in the world. Elimination programme in the country is implemented by active cooperation with state, international, private and non-governmental organisations and coordination of the Ministry of Labour, Health and Social affairs of Georgia. The first stage of elimination programme started in April 2015. Concurrently with treatment the infection control measures were activated, also large-scale screening measures started, and in 2015 by financing of various programmes, screening was conducted about to 125,000 persons.
For achievement of elimination purpose and assistance of national response to hepatitis C, hepatitis C elimination five-year strategic plan, based on recommendations issued by the WHO in 2015, was developed. In the issue of the experts’ consultations were determined plan purpose and strategic tasks. Tasks were united under 4 main purposes, to be implemented during strategy cycle in 2016-2020:
By 2020 Georgia sets as a purpose examination of 90% of persons infected with hepatitis C, treatment of 95% of those who have chronic form of hepatitis C and recovery of 95% in the issue of treatment.
24. L.IREMADZE, A.GVENETADZE, V.TANOS
PREGNANCY RATES AFTER LAPAROSCOPIC TREATMENT OF MINIMAL OR MILD ENDOMETRIOSIS – 5th YEARS OF EXPERIENCE
1Department of Reproductology, Medical Faculty, I.Javakhishvili Tbilisi State University;
2Z.Sabakhtarashvili Reproductive Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-76-79
Pregnancy rates after laparoscopic treatment are different in relation to methods of intervention and the degree of endometriosis.
Background: The objective of our study was to identify the association between minimal or mild endometriosis and infertility. Currently, definite cause-effect relationship is still controversial. The prevalence of endometriosis increases dramatically. According classical studies suggested that 25 % to 50 % of infertile women have endometriosis and that 30% to 50 % of women with endometriosis are infertile. The fecundity rate in normal reproductive age couples without infertility is estimated to be around 15% to 20%, while the fecundity rate in women with untreated endometriosis is estimated to be anywhere from 2% to 10%.
Materials and methods: This retrospective study was conducted on the basis of the Reproductive Clinic of Zurab Sabakhtarashvili. Inclusion in the study area were 704 infertile women, age 21 to 41, with excluded male factor. They underwent laparoscopy and methylene blue dye test evaluating tubal patency and staged for minimal or moderate endometriosis according to rASRM classification. Women with minimal endometriosis were treated by ablation of the visible lesions using bipolar diathermy. Patients with moderate disease underwent excision of the endometriosis implants using scissors and bipolar probe for hemostasis. The PR and time from surgery to spontaneous conception was evaluated.
Results: Among 704 laparoscopies performed 337 (48%) minimal or moderate endometriosis was diagnosed and confirmed by histopathological biopsies. Bipolar ablation of the endometriotic lesions was performed in 220 cases while in 87 patients excision of the endometriosis implants was necessary. Concomitant pathologies were noted, 22.3% had fibroids, 10.5% endometrial polyps, adhesions 10,1%. Subtle lesions like Morgan’ tubal cysts were found in 45.2% and cervical polyps in 12.5% of the cases. Among all patients with confirmed patent tubes 69/202 (34%) treated for minimal endometriosis and 53/114 (45%) with moderate disease had a spontaneous pregnancy within 40 weeks after operation. The overall PR after spontaneous conception up to 12 months post operatively was (69/220) 34.2% for the minimal endometriosis treated by ablation patients and (53/117) 45.3% for the moderate disease, endometriosis implants treated by excision.
25. S.BRUNJADZE, SH.VASHADZE, K.DOLIDZE, M.ARTMELADZE
DEMENTIA IN ONCOLOGICAL PATIENTS
Batumi Shota Rustaveli State University; Batumi Hospital “Med-Center”, Georgia.
J. Experimental and Clinical Medicine, 2018, 5, pp-79-83
Oncological diseases are one of the main causes of morbidity and mortality in the world. The problem is quite topical since the number of patients with cancer is a sensitive theme. At present, there are about 20 million people having the disease all over the world. Hence, the number of elderly people with the disease is about 10-15%. The purpose of our research was to study dementia in cancer patients. The study was conducted in the Batumi high-tech hospital (Batumi Oncological Center). 100 patients aged 25 to 75 participated in this study. Among them, the 60 patients were female and 40 were male. At the same time, only 45 patients were operated on. Of these, 16 patients were operated after chemotherapy, 4 patients after radiotherapy and 35 patients during combination therapy. 6 patients with lung cancer, 22 patients with breast cancer, 6 patients with skin cancer, 7 patients with gastric cancer, 4 patients with bladder cancer, 15 patients with ovarian cancer, 25 patients with uterine cancer were examined. In the study, three of them had the family history of dementia. We studied the patient’s medical history, physiological data, assessed their mental and cognitive functions to determine the diagnosis of dementia. We studied the patient’s medical history, physiological data, assessed their mental and cognitive functions, mini-mental state examination, and carried out a survey of family members as the patients often had difficulty precisely describing the symptoms to determine the diagnosis of dementia.
Out of 100 patients, 20% of them had mild dementia, 44% - moderate dementia and 35 % - severe dementia. Among the patients examined, dementia was not observed in 1%. Computed tomography of the brain was performed on the examined patients. Cerebral atrophy, a decrease in brain mass and volume in 34%, cortical atrophy in 60% of patients and the expansion of the ventricle system of the brain in 35% were revealed in the pathomorphological picture. The atrophic process is strongly expressed in the pariental (17%), frontal and temporal lobes (76%).
Therefore, dementia is quite common in cancer patients with severe and moderate depression, predominant in 79%. Dementia was not observed only in one case, which is very important. The researchers point out reversible dementia after chemotherapy, which is reversible after a while.
Community involvement has great importance for the treatment and psychological adaptation to the new reality of cancer patients. It is necessary to develop educational programs aimed at providing assistance from the moment of diagnosis to the end of treatment.
26. Z.NAKUDASHVILI, S.MGHEBRISHVILI, I.BARBAKADZE, T.SANIKIDZE
EFFECT OF PROSTHESES FROM VARIOUS MATERIALS ON TISSUES
OF THE ORAL CAVITY
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-83-88
We investigated the intensity of inflammatory processes (indicators of oxidative stress and balance of cytokines, a marker of apoptosis) during the use of removable prostheses from various materials.
Sixty patients were examined, who used prosthesis on the basis of Ftorax and Perflex Flexi Nylon. The parameters of oxidative metabolism and markers of inflammation of the oral cavity tissues were studied before the use of the prosthesis, after 3 days and 1 month after its establishment. For this purpose in the patients’ saliva the activity of antioxidant enzymes (SOD, catalase) was studied by spectrophotometry, the cytokine content (IL-1â, IL10) by the immuno-enzymatic method and the P53 protein content using the Cusabio test system.
An analysis of the results showed that the prothesing (Ftorax.Perflex Flexi Nylon) is accompanied by a reversible change in the oxidative and immune balance and an increase of apoptosis in the patients’ oral cavity. Intensification of oxidative metabolism and apoptosis is much less observed when using prostheses based on Perflex Flexi Nylon.
27. M. LEKVEISHVILI
TREATMENT OF A CONDITION ASSOCIATED WITH OXIDATIVE STRESS
Academy of High Teaching Education; Ltd. “Kulheiberipharm”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-88-91
Under the rusting stress is understood the information when the organism has tendency towards the rusting processes, which is caused by misbalance between the substances containing reactive oxygen and the antioxidant protection mechanism. We reckon, the rusting stress participates in many pathologic condition development, as well as the aging process enhancement.
Well-known is the curing preparation “Margali Plus” containing iron, sulphur, camomillae tincture, Helichrysum and plantain and nettle leafs. The preparation is used as hepato-protecting, desintoxicating, liver function improving, regenerative processes favoring and cholagogic substance. Its therapeutic indications are: chronic hepatitis, bile-stone diseases, cholecistites.
Before today they did not know the mentioned preparation high antioxidant activity. As a result of the research held by us we have found that the “Margali Plus” capsules when appeared in intestines (where is the acid area, in particular the mixed salty acid) the sulphur hydrogen. Physiologic role of the sulphur hydrogen in the human organism as an gasotransmitter, was found in the beginning of current century. This signal molecule plays significant role in the nervous system, heart and blood vessels system, immune system, intestines system regulation and in different organs substances interchange.
Thus, high antioxidant activity is explained by the sulphur hydrogen occurring when taking in the preparation “Margali Plus” and the highly expressed effectiveness for aging decreasing and for the treatment and prophylactic for the following pathologic condition: age-connected neurodegenerative conditions, heart and blood vessels system, eclampsy and pre-eclampsy, erective dysfunction (patent GE P 2015 6404 B).
28. G.KVARATSKHELIA, L.LURSMANASHVILI, N.LOBZHANIDZE, G.ORMOTSADZE, E.SHEKILADZE, NINA KIPIANI, T.SANIKIDZE
LIPID METABOLISM LEVEL IN POPULATION OF CHIATURA REGION WITH DIFFERENT POLLUTION
David Aghmashenebeli Georgian Univesity; Tbilisi State Medical University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-91-93
The purpose of the research was to evaluate lipid metabolism in blood of the population living in different villages of Chiatura region, with different quality of pollution level.
The villages were different from the source of ecological contamination - from manganese mine and manganese extraction level, which allows us to rank the ecological contamination (low - Khreiti, medium – Perevisa, high - Rangani).
Lipid metabolism (common cholesterol, trichlycerides) was measured through enzymatic-calorimetric method.
Based on the results of the study, the content of the total cholesterol in the highly contaminated region (Rgani) statistically exceeds its contents in relatively low contamination regions (Khreti, Perevisa). In the level of triglycerides in blood of the population of the study regions, statistically significant changes was not observed, although the trend of increase in tryglicerids content was accompanied by an increase in pollution,
The results of the research confirm the possibility of possible impact on environmental pollution on the lipid metabolism and are confirmed by the trend of increase in hypertension in contaminated areas.
29. L.TANDASHVILI, A.GVENETADZE, V.TANOS
NEONATAL UTERINE BLEEDING AS A PRECURSOR IN ADULT ENDOMETRIOSIS
Department of Reproductology, Medical Faculty, I.Javakhishvili Tbilisi State University;
Z.Sabakhtarashvili Reproductive Clinic, Tbilisi, Georgia; University Nicosia, Medical School, Cyprus
J. Experimental and Clinical Medicine, 2018, 5, pp-93-97
We elaborate on a new theory to explain pelvic endometriosis, including endometriosis in pre-menarcheal girls, based on the finding that the neonatal endometrium can display secretory activity immediately after birth and, in some cases, changes analogous to those seen at menstruation in adults.A new theory was outlined by I.Brosens and G.Benagiano, in 2013, based on the extrapolation that endometrial stem cells may become disseminated in the pelvis at the time of neonatal uterine bleeding (NUB). These neonatal endometrial stem cells may in turn be responsible, through a variety of mechanisms, for early-onset endometriosis.
30. D.BAKHTURIDZE, Z.DABRUNDASHVILI, T.MAGLAKELIDZE, T.CHIBURDANIDZE, K.MARDALEISHVILI
VOCAL CORD PARALYSIS. ETIOLOGY AND CLINICAL FEATURES
Oncologic Scientific Centre, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-97-101
Vocal cord paralysis is the result of the pathologic process or processes which cause the damage of vagus nerve or its branches - recurrent laryngeal nerve and superior laryngeal nerve. These branches innervate larynx; which is the important part of the upper airways, and is responsible for breathing and voice production, therefore the disruption of the larynx innervation causes the vocal cord paralysis. This at different level affects the functions of voice production and airway passage.
The clinical features of the vocal cord paralysis vary from slight changes in voice quality to life threatening airway obstruction.
The vocal cord paralysis is caused by multiple reasons, it could be caused by problem in central nervous system or in periphery.
In choosing the optimal way of treatment, it is crucial to identify the etiology of this problem.
31. N.MGALOBLISHVILI, M.GOTUA
DIAGNOSTIC VALUE OF EXHALED NITRIC OXIDE (FENO) - TYPE 2 BIOMARKER OF ASTHMA
Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-101-106
Background: With the increasing level of knowledge, asthma is now being considered as a markedly heterogeneous disease. There is growing interest in the use of Type 2 biomarker such as Fraction of Exhaled Nitric Oxide (FeNO) in asthma diagnosis and management. FeNO has been shown to noninvasively identify Type 2 asthmatic adults and children from healthy subjects with high sensitivity and specificity. The aim of our study was to evaluate diagnostic value of FENO levels as Type 2 asthma biomarkers in suspected asthma patients in Georgian population. Material and Methods: A total of 262 nonsmokers, steroid-naive subjects were investigated. These patients were free from symptoms of viral infection for at least two weeks before. FENO concentration at an expiratory flow rate of 50 mL/sec was determined by using a portable device (Niox Vero; Aerocrine AB; Stockholm, Sweden). Clinical questionnaire, FENO measurements and spirometry were performed according to EAACI/AAAAI and ATS/ERS recommended methodologies. Results: The highest median FENO levels revealed in Eosinophilic Asthma (122.50+28.39) compared to Non-Type 2 asthma phenotypes (p-0.0001). An ROC analysis showed the area under the ROC curve ROC AUC=0.853(95%CI: 0.733-0.974), which means that prognostic accuracy of this diagnostic test is high. In studied patients FeNO identified Type-2 asthma with sensitivity of 98% [95% CI% 94.75 –99.10] and specificity of 71% [95% CI% 44.04–89.68]. Conclusion: Our study revealed that FeNO identifies Type-2 asthma with high sensitivity and specificity in Georgian population. Thus, FENO measurement may be an effective auxiliary tool for diagnosis type-2 asthma phenotype.
32. D.BAKHTURIDZE, Z.DABRUNDASHVILI, T.MAGLAKELIDZE, T.CHIBURDANIDZE, K.MARDALEISHVILI
THE METHODS OF TREATMENT OF VOCAL CORD PARALYSIS
Oncologic Scientific Centre, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-106-111
Vocal cord paralysis is one of the main problems of the modern medicine, because it could be the reason of acute or chronic airway insufficiency. The clinical features of the airway insufficiency vary from slight problems with breathing after physical exercises to life threatening airway obstruction. Such patients need to be treated surgically, to restore the function of their larynx. Tracheostomy usually is the primary aid to such a patients, afterwards the next step of treatment is chosen individually.
The methods of treatment of bilateral vocal cord paralysis is divided into two groups: static and dynamic. Despite the fact that the treatment methods of the vocal cord paralysis have been much more improved and sophisticated from the 1922 when they were first described, still none of them is good enough to solve the problem completely, all of them have many disadvantages. Since 2000 in our clinic we are using several methods of treatment of this problem, we are improving them. Nowadays we have modified new version of the treatment, which provides a sufficient airway passage, with a minimal disadvantages.
33. M.GARUCHAVA, G.PARULAVA
STUDY PECTINICS IN SOME FOOD CROPS
Georgian State University of Physical Education and Sports, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 5, pp-111-114
The composition of pectinetics and some physical-chemical characteristics identified by hydrolysis and extracting from stone crops and berry crops.
Extracts with pectin maximum extraction from selected cultures are selected. The optimum temperature for complete hydrolysis is determined.
The physical-chemical characteristics of the pectin studied are indicative of the fact that the gel of these substances can be used as a substance and lubricating substance in the food industry and medical practice.