J.EXPERIMENTAL & CLINICAL MEDICINE
2018, ¹ 2
1. Z.MANIZHASHVILI, G.AKHALADZE, N.LOMIDZE, G.KOPADZE, I.TSERETELI
MODERN PRINCIPLES OF THE ACUTE PANCREATITIS MANAGEMENT
Tbilisi State Medical University, Department of Surgery ¹1; TSMU the First University Clinic, N.Kipshidze Central Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.9-20
The diagnosis of acute pancreatitis (AP) is most often established by clinical symptoms and laboratory testing and contrast-enhanced computed tomography (CECT) and/or magnetic resonance imaging ( MRI) of the pancreas should be reserved for patients in whom the diagnosis is unclear or who fail to improve clinically. Patients with organ failure and/or the systemic inflammatory response syndrome (SIRS) should be admitted to an intensive care unit or intermediary care setting whenever possible. Aggressive hydration should be provided to all patients, unless cardiovascular and/or renal comorbidites preclude it. Early aggressive intravenous hydration is most beneficial within the first 12-24 h, and may have little benefit beyond. Patients with AP and concurrent acute cholangitis should undergo endoscopic retrograde cholangiopancreatography (ERCP) within 24h of admission.
Routine use of prophylactic antibiotics in patients with severe AP and/or sterile necrosis is not recommended. In mild AP, oral feedings can be started immediately if there is no nausea and vomiting. In severe AP, enteral nutrition is recommended to prevent infectious complications, whereas parenteral nutrition should be avoided. In stable patients with infected necrosis, surgical, radiologic, and/or endoscopic drainage should be delayed, preferably for 4 weeks, to allow the development of a wall around the necrosis.
2.1L.BERDNIKOVA, 2L.BARAMIDZE, 2D.TABIDZE
MODERN APPROACHES TO ATTRACTION OF THE PATIENTS AND FORMATION OF THEIR LOYALTY
St.Peterburg Consulting Manager, Russia; 2TSMU Public Health, Management, Policy and Health Economics Department, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.20-22
Agree, the task of the doctors and the specialists is not just rendering service, treating and watching over the patients’ health status, today, in order to be successful in one’s own practice, one has to pay much attention to communication with the patients, methods of their attraction and increase of their loyalty.
Better give him as much information as possible during the first visit, schedule a recommended treatment plan, tell him about prevention in home conditions - help your patient right now, without any imposed sales.
Thus you will be able to constantly work not only on your own qualification, but also work on increase of the patients’ loyalty.
Using technology of communication with the patients, working on increase of the patients’ loyalty, you really can increase your own workload and income for several times.
3. N.KINTSURASHVILI, G.GALDAVA
THE EPIDEMIOLOGICAL CHARACTERISTICS OF TINEA PEDIS
Tbilisi Iv.Javakhishvili State University, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.22-27
Tinea pedis is one of the most common diseases in the world, which can infect people of all ages and sex. Approximately 70% of the world’s population has suffered this pathology, the most common etiological agents are dermatophytes. The epidemiological condition of disease in different countries is distinguished by specificity, which is primarily dependent on the economic development of the particular state and the social level of its population.
In this work, we will discuss epidemiology of Tinea pedis based on studies conducted in different countries. We also compare the characteristics of the spread of the disease in children and adults.
The review of the literature has been carried out using the “ScienceDirect”, “Scopus” and “PubMed” scientific bases in order to define relevant scientific works.
As a result of a thorough review of the received data, the increase in the spread of Tinea pedis in the world has been defined. The conducted studies have shown that the prevalence Tinea pedis in children is lower compared to adults, and is more common in men than in women. According to the studies, accompanying diseases do not affect the development of Tinea pedis. Several risk groups have been identified: athletes, miners, soldiers, parishioners of mosques, homeless people, etc. The spread of Tinea pedis has changed due to changes in migration, lifestyle and socio-economic conditions. The global spread of fungal infection happened in the second half of the twentieth century, especially the one of T.Rubrum-shape. This was caused by an increase in urbanization, as well as intensive development of gyms and fitness halls.
4. D.TAVDIDISHVILI, R.SAKANDELIDZE, M.PKHAKADZE, T.GRDZELIDZE
INFLUENCE OF PRODUCTS OF BEE POLLEN AND BEE BREAD ON THE HEMATOLOGICAL INDICATORS OF ORGANISM
Akaki Tsereteli State University, Kutaisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.27-30
Influence of Honey, Bee Pollen and Bee Bread containing food products on haematological indicators of experimental rats has been studied. It is shown that their using increases the hemopoesis process, increases the total number of erythrocytes and hemoglobin; Improves quantitative content of blood plasma, albumin and globulin. The results indicate that using the new products are important for human health, for anemia prophylaxis and strengthen the immune system.
5. Z.DABRUNDASHVILI, D.BAKHTURIDZE, K.MARDALEISHVILI
TACTICAL MISTAKES OF DIAGNOSIS AND TREATMENT METHODS OF CANCER OF THE SUBGLOTTIC LARYNX
Georgian Oncological Scientific Center, Tbilisi
J. Experimental and Clinical Medicine, 2018, 2, pp.30-34
Despite the fact that the cancer of the subglottic larynx is only 3-5% of the total number of patients with laryngeal cancer, it is with this tumor localization that the greatest number of diagnostic errors occur (60-70%). First of all, this is due to the difficult visibility of the subglottic department, which makes it difficult to establish an accurate diagnosis. In addition, the symptoms of cancer in the subglottic larynx department are often similar to those in chronic laryngitis, angina and other non-oncological diseases.
The lack of competence of the doctors of the polyclinic network and the oncologic alertness of otorhinolaryngologists leads to an unjustifiably long and multi-stage examination, which increases the number of patients with common processes (stage III-IV). It should be noted that, despite the use of numerous methods of examination, there are still cases of inadequate evaluation of the incidence of the tumor in the subglottic larynx, which leads to incorrect choice of treatment methods, reassessment of the possibilities of radiotherapy and, as a consequence, a decrease in the effectiveness of treatment.
6. G.KVARATSKHELIA, E.TIKARADZE, I.CHKHIKVISHVILI, N.LOBZHANIDZE, M.BULEISHVILI, NANA KIPIANI, NINA KIPIANI, T.SANIKIDZE, G.ORMOTSADZE
BLOOD REDOX-BALANCE AS A SENSITIVE TEST-SYSTEM FOR ASSESSING THE HEALTH STATUS OF THE POPULATION
David Aghmashenebeli Georgian University, Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.34-39
The purpose of our study was assesment redox-balance of the population of various points in the Chiatura district in Georgia (both sexes), living in villages characterized by varying degrees of environmental pollution. This indices, characterized by different degrees of environmental contamination (Khreyiti - low, Perevisa - medium, Rgani - high), has been studied. The redox blood balance was determined by means of modified DPPH (2.2-diphenyl-1-picrylhydrazyl) test.
The results of comparison of the degree of ecological contamination in the villages and the level of the antioxidant potential of inhabitants’ blood show the particularly low value of the blood redox potential in residents of Rgani. That should be due to the high level of contamination of this area and the inactivation of the antioxidant protection system of the body. A significant increase in the redox balance of blood in the residents of Perevisa indicates the presence of body’s redox-active processes inductor and sufficiently high antioxidant potential of blood in conditions of moderate contamination.
It was concluded that the blood redox system is a sensitive marker reflecting both the degree of environmental pollution and the compensatory ability of the body’s antioxidant defense system. The results obtained suggest the method used to determine the redox blood balance as a sensitive test system for population-based health research.
7. G.JANASHIA, Z.CHKHAIDZE, M.NACHKEPIA, K.BESELIA
ASSESSMENT OF THE RISK OF PERIOPERATIVE COMPLICATIONS IN CORONARY ARTERY BYPASS GRAFTING.
Iv.Javakhishvili Tbilisi State University, Tbilisi Heart and Vascular Clinic,
Aleksandre Aladashvili Clinic, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.39-42
The aim of our study is to assess the risk of periodic complications in coronary bypass surgery.
A study was conducted on the analysis of clinical material of 402 patients aged 37-75 years. Uncomplicated patients were compared with those patients who had complications from the list in any case - intraoperative shock, bleeding, heart failure, dissection, firilation, pneumonia, other pulmonary complications, renal failure.The comparison between the groups was carried out with the Fisher criterion, assessing the risk of complications with a regressive analysis. Statistical analysis was performed using SPSS 22.
Results. The chances of periodic maneuver complications increase the chances: Operation using op pump - OR = 2.33 (95% CI: 1.35-4.04); Age <45 years - OR = 3.12 (95% CI 1.29-7.55); violation of regional myocardial contraction - OR = 3.72 (95% CI: 1.83-7.56); Deficiency of the tricuspid valve - OR = 2.92 (95% CI: 1.09-7.81); Transient ischemic attack - OR = 15.66 (95% CI: 2.85-86.18); Heart shock in history - OR = 5.42 (95% CI: 1.29-22.71).
Conclusion. Predictors of periodic shunt complications include: surgery with artificial blood circulation less than 45 years, myocardial region - infectious, defective threshold valve, transient ischemic attack, heart shock in history.
8. S.TATISHVILI, R.JORBENADZE, G.KAVTARADZE
DEPRESSIVE SYMPTOMS IN PATIENTS WITH ACUTE CORONARY SYNDROME
Tbilisi State Medical University, Department of Internal Medicine #3;
G.Chapidze Urgent Therapy Center, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.42-47
Among non-cardiac comorbid conditions, psychosocial risk factors have been associated with increased morbidity and mortality and poor prognosis in patients with ACS. The aim of our study was to identify factors associated with depressive symptoms in patients with acute coronary events (unstable angina/ non-ST segment elevation myocardial infarction).
Depression screening was performed in the G.Chapidze Center. Main inclusion criteria in the study were acute coronary events – non-ST segment elevation myocardial infarction or unstable angina. The total number of participants was 84. Descriptive statistical tests were used for the calculation of frequencies, means and standard deviations. A Chi-square test was applied for categorical variables in order to establish difference between groups. The independent T-test was used to compare means for numerical variables.
Most of the participants had multiple coronary risk factors – obesity, diabetes and arterial hypertension. A higher depressive score was found in women and in patients with reduced ejection fraction.
Conclusion: Identification of depressive symptoms in patients with acute coronary syndrome will contribute to improved prognosis and reduction of mortality.
9. M.GONGADZE, N.GOGIA, M.MACHAVARIANI, M.ENUKIDZE, I.CHKHIKVISHVILI
INFLUENCE OF FRENCH MARIGOLD (TAGETES PATULA L.) FLOWER EXTRACT ON OUTCOME OF DIABETIC PREGNANCY
TSMU V.Bakhutashvili Institute of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.47-53
Diabetes type 1 and type 2 in pregnancy results in increased risks to both the mother and child. Beside the high rate of spontaneous abortions, babies are at increased risk of congenital malformations, perinatal mortality, macrosomia and related complications, which is thought to be attributable to poor glycemic control. The aim of our current study is to evaluate whether administration of French Marigold (Tagetes Patula L.) flower extract gives us possibility to avoid or reduce undesirable outcome by modulating mechanisms underlying diabetes-induced pregnancy loss. To clarify this question, we performed the study on streptozotocin (STZ) treated FVB female mice. To investigate influence of TPE administration on diabetes teratogenic stimuli in mice depending on severity of diabetes, mice were injected 19,95 mg per mice TPE tree times during implantation period (day 4,6 and 8 of pregnancy).
Therefore, pregnancy rate and spontaneous abortion were investigated on day 9 of pregnancy, when implantation period is completed. To characterize influence of TPE administration on expression of TNFα and NO in the uterine cells of diabetic mice, we analysed uterine lysates of diabetic mice collected on days 5 and 7 of pregnancy, encompassing the period of implantation. Also was investigated diabetes induced congenital malformations on day 15 of gestation.
In conclusion, the data presented in this article suggest that NO and TNFα play a crucial role in the pathogenesis of diabetes- induced pregnancy loss. Also, TPE administration had a strong inhibitory effect on nitric oxide and pro-inflammatory cytokine generation in uterus. Therefore, supposedly, French Marigold Extract reduces spontaneous abortion and resorption rate; Increases embryos weight and reduces diabetes-induced embryopathic stress.
10. I. Lomsianidze, K. Gabunia, N. Endeladze, M. Kukhalashvili
Food Products High in Nitrates and the Impact of Methemoglobin
On the Organization of Anxiety Behavior of Animals
Akaki Tsereteli State University, Kutaisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.53-58
Mineral fertilizers have been used in Europe since 1930, but the use of nitrogenous fertilizers has its disadvantages as well. It can cause nitrate accumulation in food. In the digestive system of the human and animal organisms nitrates convert to nitrites and with blood hemoglobin produces methemoglobin-MHB. It is accompanied by the increased anxiety in animal behavior.
The formation of methemoglobin in the blood and the intensification of behavioral anxiety were studied when feeding animals (white laboratory rats) with food high in nitrates.
The content of nitrates in food products was determined by the ionometric method. Methemoglobin was determined by Gorn’s method using photoelectrocolorimeter. Anxiety behavior was studied using an elevated plus maze.
The study results are as follows: 1. On the market there are a number of products that are rich in nitrates. 2. In rats, methemoglobin has been found in 10%. 3. Intensification of anxiety behaviour has been observed.
11. K.MANJGALADZE1, G.TEDORASHVILI1, T.MUZASHVILI1, M.GACHECHILADZE2, J.SKARDA2, G.BURKADZE1
PROBLEMATIC ISSUES OF TUMOR MICROENVIRONMENT ALTERATIONS IN THE PATHOGENESIS OF CERVICAL INTRAEPITHELIAL NEOPLASTIC LESIONS
1Tbilisi State Medical University, Tbilisi, Georgia;
2Palacký University, Olomouc, Czech Republic
J. Experimental and Clinical Medicine, 2018, 2, pp.58-64
The frequency and mortality rates from cervical cancer is dependent on the presence of screening programs and HPV vaccination. In Georgia, compared to the statistics of 2014, the cervical cancer incidence has decreased and it became the 5th most common cancer. It is well known that only 10% of HPV infected women develop cervical intraepithelial neoplasia and subsequently, subset of them, cervical cancer. It is suggested that the carcinogenic potential of HPV virus is influenced by cervical microenvironment. Tumor microenvironment consist of different population of T and B cells, macrophages and dendritic cells. The synchronic or subsequent activity of these cell types is suggested to play an important role in HPV elimination and/or the development of HPV induced cervical carcinogenesis.
Despite the ample of original studies in this field, there is no clear evidence about the role of tumor microenvironment and its cellular constituents in the development of cervical intraepithelial neoplasia and cervical cancer after HPV infection. Dominant factor or the collection of factors, from the known microenvironmental characteristics, which cause the failure of immune response against HPV is not yet identified.
12. N.GONGADZE, L.GABUNIA, D.DELIBASHVILI, M.GVISHIANI, T.MAKHARADZE
EFFECTS OF FOODS AND THEIR ACTIVE INGREDIENTS ON METABOLISM AND
EFFICACY OF DRUGS
Department of Medical Pharmacology of the Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.64-71
According to the World Health Organization Pharmaco-Epidemiological studies, the mortality from complications associated with drugs is in the V-VI place in developed countries after cardiovascular, cancer, trauma, poisoning and respiratory diseases.
Side effects are one of the most frequent problem for the doctor and this is often the reason for hospitalization; one of the most important reasons for the development of pharmacological complications are interaction of drugs and their combinations with food.
Individualization of human treatment or personalization excludes random combinations, as well as the use of random combinations of drugs and with food, in order to avoid unexpected complications of therapeutic interventions.
In the last decade, researchers, authors of new medicines, regulators pay much attention to personalized medicine, which implies the treatment with appropriate diet, take into account frequent use of alcohol and tobacco during prescription of treatment.
The best prescription (or the best treatment), the choice of the rationale using the most effective and safe drugs or their combinations, the take into account and anticipate all the possible interactions between drugs and between drugs and foods is the most difficult and complex process in the activity of a doctor.
Each prescription of drugs or their combinations should be well thought out and highly motivated, like any surgical intervention, because our main goal is to help and not to harm!!!!
THE FIRST GRADE CHILDREN PHYSICAL DEVELOPMENT INDICATORS REVIEW
Akaki Tsereteli State University. Kutaisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.71-74
The study is subject to children who belong to a group of practically healthy groups. We observed the first grade pupils at three Public Schools in Kutaisi, whose age is 6-7 years. The number of researchers who made a thorough analysis are 105. There are 55 girls and 50 boys. At this stage only physical development indicators are analyzed.
In comparison with the obtained data from different data, we find that the physical characteristics of the first-year-old children are not far behind the average data and Physical development in this age category is going on without interruption.
Of course, for the complete and reliable data, we need a lot of children who have studied and we can put out more exhaustive information.
14. T.KIKNADZE1, G.TEVDORASHVILI1, T.MUZASHVILI1, M.GACHECHILADZE2,
PROBLEMATIC ISSUES IN THE HISTOPATHOLOGICAL AND MOLECULAR CHARACTERISTICS OF LEIOMYOMAS
1Tbilisi State Medical University, Tbilisi, Georgia;
2Palacký University, Olomouc, Czech Republic
J. Experimental and Clinical Medicine, 2018, 2, pp.74-81
Uterine leiomyomas represent one of the most common pathology of genital tract in 50 year old females, with the incidence of 70%. The well known risk factors of leiomyoma are black race, nulliparity and obesity. Estrogen and progesterone, as well as inherited pathology of myometrium play an important role in leiomyoma aetiology and pathogenesis. Another important factors are TGF-β, bFGF, EGF, other growth factors and prolactin. Also, aromatase and tumor specific chromosomal abnormalities, such as 7q deletion, 12q15, 6p21 and others are playing a role. Despite the high number of original studies, nowadays, there is no clear information about the mechanisms of the origin and development of leiomyomas. The mono and polyclonality of several leiomyomas originated in one uterus, the prognostic markers and the markers of relapse after myomectomy are also not clear. Limited information exist about the proliferation and apoptotic markers and the role of stem cells in the development and progression of leiomyomas.
15. L.BUGHADZE, L.ZHORZHOLIANI, N.MANJAVIDZE
CLINICAL IMPORTANCE OF VITAMIN D DETERMINING IN CHILDREN WITH BRONCHIAL ASTHMA
Georgian David Aghmashenebeli University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.81-85
Asthma prevalence is increasing, especially in children. Latest research suggests vitamin D significance possible importance in allergic diseases. The aim of our research is to estimate the importance of determining blood serum vitamin D in children with bronchial asthma.
METHODS: The study was performed in 70 children aged 6 -15 years, including patients with asthma (n=50) and healthy children ( n=20), who referred Sachkhere medical center for visit.
Status of asthma control in basic group was classified as controlled (n=31) and uncontrolled (n=19). Children underwent serum vitamin D level test for study.
RESULTS: 48% of patients In the group- controlled asthma (n=15) had vitamin D deficit, and in 52% of cases (n=16) was confirmed vitamin D insufficient. In the group –uncontrolled asthma - 5% of patients (n=1) had Vitamin D insufficiency in blood serum. In 95% (n=18) patients vitamin D level was significantly low <20 ng/ml. 20 % (n=4) of patients in control group, had normal level of vitamin D in blood serum, while in 80% (n=16) of patients were confirmed vitamin D insufficiency. There was not found vitamin D deficiency in control group participants.
Using the multivariate logistic regression analysis, the presence of asthma was associated with reduced level of vitamin D (OR =1.35, 95% CI (1,14-1.58) P = 0.011; X²=6.78; F-0.022).
CONCLUSION: The presence of vitamin D deficiency effectively predict increased risk for childhood asthma; Study revealed that the majority of patients with asthma had vitamin D deficiency, compared to control group participant.
16. M.SVANIDZE, Z.BERISHVILI, M.KUPARADZE, N.GARUCHAVA, N.MEGRELISHVILI
SOCIAL ASPECTS POST-ACUTE INFRACTION REHABILITATION
Caucasus International University; Teaching University “Geomedi”;
Tbilisi State Medical University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.85-89
The objective of the study was the opinion of patients about quality of earli rehabilitation program after acute infarction and co-financing by the goverment, for wich 191 patients were interviewed from this program. Most of the interviewed patients did not have information on the modern methods of rehabilitation, which makes it necessary to increase the awareness of the population.. Despite of the high percentage of co-financing of the program by the goverment, the most of the patients are incapable of paying the remaining amount, part of the patients speaks about the shortcomings of the provided service and the medical personnel service, which requires a deeper and thorough study.
GENETIC FEATURES OF TNF-A
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.89-92
The molecular genetics opens the new horizons in modern medicine. especially now when high prevalence of several diseases among various groups of population is observed. It is reported extremely high interleukin genes polymorphism of tumor necrosis factor (TNFa). In this article we discuss that genetic variations in MHC molecules affect resistance and susceptibility to some diseases.
18. I.ZARNADZE, L.BARAMIDZE, Z.SIKHARULIDZE, D.TABIDZE
FAMILY PLANNING IN GEORGIA, AS A PUBLIC HEALTH AND SOCIOLOGY JOINT PROBLEM
Tbilisi State Medical University, Public Health, Management, Policy and Health Economics Department, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.92-94
TSMU Dept, as a result of carrying out a research, once again shows that the family planning in Georgia and demographic situation is treated as a public health and sociology joint problem. The necessity of the public is to inform them about the family planning and childbirth regulations. The combination of socioeconomic and childhood is an unconditional way to improve the demographic situation.
Solving all of the above-mentioned problems is inconceivable for the joint action of governmental and non-governmental organizations.
19. 1T.MURTAZASHVILI, 2N.MITAGVARIA
STUDY OF ANTI-INFLAMMATORY ACTION OF THE CAMELYN EYE DROPS
1Tbilisi State Medical University, Faculty of Pharmacy; 2Ivane Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.94-99
Anti-inflammatory action of Camelyn eye drops has been studded on animals (white mice and rabbits). With lipopolysaccharide – on endotoxin-induced uveitis by experimental model. Everyday examination of animals eye structures was conducted with bio-microscope (Colemeter, Cooling Tech Grenada) in control groups as well as in experimental groups. Principal differences were detected in histological material of control group animals: in material without Camelyn was shown significant inflammation processes. Treatment with Camelyn fully restored corneal epithelialization. Received study results gives us an opportunity to conclude that Camelyn eye drops can be recommended for treatment and prevention of eye uveal tract any part inflammatory diseases.
20. I.CHKHIKVISHVILI1, N.GOGIA1, D.CHKHIKVISHVILI2, M.ESAIASHVILI1
ANTIOXIDANT ACTIVITY AND POLYPHENOLS CONTENT IN THE BEAN AND CEREALS CULTURES
1TSMU, V.Bakhutashvili Institute of Medical Biotechnology;
1Company “Bioantioxidanty”, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.99-104
Among the studied fifteen plant cultures the highest antioxidant activity and the total polyphenols content in the rape seeds (48±0.6 mg/g, R=500) Brassica oleifera moench L. Phaseolus vulgaris of “Gurula”, (24±0.5 mg/g, 250) had the highest antioxidant activity and the total polyphenols content in Leguminous plant cultures. The correlation between the total polyphenols content and antioxidant activity is observed in the ten bean samples tested, except for „Udelebi“ and „Mertskhala“ bean samples, in which, with a small amount of polyphenols, high antioxidant activity. In other Leguminous cultures, antioxidant activity and the total polyphenols content were studied in Chickpeas Cicer arietinum> Lentils Culinaris lentils> Peas Pisum sativum L> Flax Linum usitatissimum L.> Green peas> Soybean Glycine max (L.)
The highest antioxidant activity in cultures of Cereal crops are found in buckwheat culture (7.8±0.4 mg/g, 142) Fagopyrum esculentum. Also has high barley indices Hordeum vulgare, rye Secale Cereale and oats Avena sativa, but by the total polyphenols content, the rye sample is superior to the rest. Polyphenols and antioxidant activity of yellow corn (8.5±0.06 mg/g, 90) are almost twice as large as white (4.7±0.09 mg/g, 51) Zea Mays. The lowest antioxidant activity in wheat, but in the content of polyphenols (5.5±0.04 mg/g), it takes not the last place. The most significant indicators of antioxidant activity in samples of millet Panicum miliaceum L. and rice Oryza L .
21. Z.DABRUNDASHVILI, D.BAKHTURIDZE, K.MARDALEISHVILI
THE NEED FOR PREVENTIVE TREATMENT OF PRE- AND PARATHRACHEAL METASTASIS IN PATIENTS WITH SUBGLOTTIC CANCER OF THE LARYNX WITHOUT A CLINICAL MANIFESTATION OF METASTASIS
Oncological Research Center of Georgia, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.104-108
The study was carried out with the patients who had subglottic cancer of larynx, who clinically did not have metastasized damage in the pre-and paratracheal zones at the time of their initial addressing in the hospital.
For the prevention of metastasis, all patients underwent extended-combined laryngectomy with lymphodissectionof the VI zone of the neck and hemithyreoidectomy with the bronchial side of the tumor. Since, 12 out of 22 patients were found to have metastases in lymphatic glands and 2 patients had in thyroid glands, such type of surgical interference was reasonable. Because a significant number of patients usually die not with the primary tumor recurrence, but in response to regional metastasis, this level of operation which significantly reduces the frequency of metastasis, is very important for prevention of metastasize.
DNA MEHYLATION PROFILE IN CHRONIC INFLAMMATION
TSMU V.Bakhutashvili Institute of Medical Biotechnology; Department of Molecular and Medical Genetics, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.108-112
Epigenetic modifications include DNA methylation, histone modifications, histone variants and noncoding RNAs. They are influenced by environmental exposure, such as life-style, aging, infections, and other environmental factors and have been shown to be altered in many complex diseases including inflammatory diseases. Epigenetic pathways involved in the modulation of inflammatory and anti-inflammatory genes are crucial for immune responses. This review briefly summarizes the reported changes in patterns of DNA methylation during the inflammatory response.
23. Kh.SOKHADZE, J.KRISTESASHVILI
FEATURES OF TREATMENT OF WOMEN WITH PROLACTINOMA AND REPRODUCTIVE PROGNOSIS
Iv.Javakhishvili Tbilisi State University, Medical Faculty, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.112-117
A literature review of English-language articles was conducted using the internationally recognized scientific bases. Scientific works published during the last 5 years have been reviewed which focus on the peculiarities of treatment in women with prolactinoma and their reproductive prognosis.
Based on the literature review, it has been established that microadenomas well respond to pharmacological therapy; the reproductive function may be restored.
According to modern literature data, diagnosis of microadenomas is conducted insufficiently that is very important in the prevention of formation of macroadenomas. In case of macroadenoma, surgical treatment is mostly inevitable, because a tumor pressures on the tissues near the brain, in particular, on the optic nerve. This can result in visual deterioration and the process becomes irreversible.
The radiological method of treatment of pituitary adenomas is used relatively rare.
Based on a thorough review of the received data, it was found that the effectiveness of treatment with dopamine agonists is high; they are characterized by reduction of prolactin levels in the blood, decrease in pituitary adenoma size, elimination of hypogonadism associated with hyperprolactinemia, and restoration of fertility.
FINDING OF CORRELATION BETWEEN MRI PERFUSION PARAMETERS AND TUMOR GRADES IN THE STUDY OF CEREBRAL GLIOMA
David Aghmashenebeli Georgian University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.117-122
Perfusion MRI can be used to assess the hemodynamic properties of a tumor, such as the volume or flow of tumor blood, oxygenation, vessel size and vascular permeability. In primary malignant gliomas, vascular morphology is a critical parameter in determining the malignant potential of neoplasms and the patient’s survival.
To assess the accuracy of the diagnosis of CBV and CBF values obtained from the MRI DSC study to evaluate cerebral glial tumors and to evaluate the correlation between perfusion parameters and tumor malignancy levels.
All 23 patients included in the studies were diagnosed with a primary or secondary brain tumor in 2006,2007 and 2017. Study was performed at Scientific Institute of Clinical Medicine (Tbilisi, Georgia) and Arturo Soria Hospital (Madrid, Spain).
The differences in rCBVt values were statistically significant among all tumor grades. Correlation analysis using Pearson was as follows: rCBVt and tumor grade, r=0.774; rCBFt and tumor grade, r=0.417; rCBVt and rCBFt, r=0.605.
CBVt showed high discriminative power in distinguishing all tumor grades. rCBVt correlated strongly with tumor grade.
USING MRI MORPHOLOGICAL PARAMETERS AND DIFFUSION TENSOR PARAMETERS FOR DIFFERENTIATION OF TUMOR RECURRENCE AND RADIATION DAMAGE IN PATIENTS WITH GLIOBLASTOMA
David Aghmashenebeli Georgian University, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.122-126
Multiform Glioblastoma (GBM, IV class) is the most malignant form of the primary brain tumor, characterized by frequent relapses after treatment. Non-invasive imaging is an important component of planning and monitoring the treatment of brain tumors. Unfortunately, tumor recurrence and radiation damage (RI) in patients with GBM have similar manifestations in control MRI, which makes it difficult to choose the most appropriate treatment plan.
The objective was to develop a method to discriminate between GBM tumor recurrences and RI using a multiparametric characterization of the tissue incorporating conventional MRI signal intensities (T2-weighted (T2w) and fluid attenuated inversion recovery (FLAIR)) and diffusion tensor imaging parameters (fractional anisotropy (FA) and radial diffusivity (RD)).
Twelve patients with glioblastoma were recruited for the study. The study was performed at Arturo Soria Hospital (Madrid, Spain). Patients were included for this study if they met the following criteria: histologically proven cancer before radiation and chemotherapy, magnetic resonance imaging findings on follow-up clinical MRI and clinical presentation consistent with glioblastoma. Patients underwent a perfusion CT scan and a 3 Tesla MRI.
There was a strong positive correlation between MD and RD (r = .98, p< .01) in both the high permeability (tumor) region and in the low permeability (RI) region (r = .98, p< .01). The low permeability (RI) region also produced a strong negative correlation between FA and RD (r = - .76, p< .05) and a strong positive correlation between T2w signal intensity and FLAIR signal intensity (r = .89, p< .01).
26. M.PATARAIA, B.KORSANTIA, M.AKHVLEDIANI, Ts.GAGNIDZE
INFLUENCE OF THE ZEOLITE DIET ON IMMUNOLOGICAL INDICATORS
AND ENDURANCE OF WHITE MICE IN ACUTE PHYSICAL OVERLOADING
Tbilisi State Medical University, V.Bakhutashvili Institute of Medical Biotechnology;
Georgian University of Physical Education and Sport, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.126-132
Studies have shown that the zeolite diet contributes to a significant improvement in immune homeostasis, not only in intact white mice, but also in immunodeficiency caused by physical overload. In a one-stage experiment (on the 11th day of the experiment), it was shown that intact (non-diabetic) mice swam an average of 142.3 min, whereas the limiting time of “zeolitic” mice, i.e., swimming to failure, increased to 184.5 minutes (almost 30%, the difference is reliable, p<0.02). This is a good example of the fact that under conditions of optimizing the state of the immune system with zeolites, it is possible to achieve a reliable immunorehabilitation of the organism and, as a consequence, to increase the resistance of animals to physical overloads.
27. S.LILUASHVILI, T.KITUASHVILI
DERMATOLOGY LIFE QUALITY INDEX IN PSORIASIS PATIENTS
Iv.Javakhishvili Tbilisi State University, Medicine Dept.; National Center of
Dermatology and Venereology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.132-135
Psoriasis is a chronic, inflammatory and relapsing psycho-dermatological condition that affects patients of all ages and both gender. Psychological factors can trigger and/or exacerbate skin lesion, relapse of disease can be a source of stress and can worsen patient’s Quality of Life.
The aim of this study was to illustrate connection between Psoriasis Area and Severity Index (PASI) and Dermatology Lifequality Index (DLQI).
Methods:108 patients with psoriasis were enrolled in the study. The severity of psoriasis was assessed by the Psoriasis Area and Severity Index (PASI), the Dermatology Life Quality Index (DLQI) was used for quality of life assessment.
The results of the study indicate the relationship between Psoriasis area and Severity Index and quality of life. Psoriasis is a distressing disorder that significantly impairs quality of life. There is a close link between Psoriasis area and Severity Index and quality of life. Patients with severe psoriasis (PASI>10) have extremely low indicator of Dermatology Life Quality Index. As a result, future management of psoriasis may require involvement of multidisciplinary teams that help patients to manage physical, psychological and social aspects of the condition.
28. 1IATSYNA O.I., 2VERNYGORODSKYI S.V, 1KOSTYEV F.I.
THE IMPACT OF PHARMACOCORRECTION ON MORPHOGENESIS OF OVERACTIVE DETRUSOR AND URODYNAMIC STRESS INCONTINENCE
1Odessa National Medical University, Ministry of Public Health;
2National Pirogov Memorial Medical University, Vinnitsa, Ukraine
J. Experimental and Clinical Medicine, 2018, 2, pp.135-144
Purpose of the study was to provide histo- and immunohistochemical evaluation of the quantitative composition of bladder wall connective tissue under stress urinary incontinence and its overactivity prior- and post-treatment with Mirabegnon, Spasmex, Quercetin and their combinations with testosterone and estradiol.
We studied main components of the connective tissue bladder wall frame on the experimental models of overactive bladder (OAB) and stress urinary incontinence (SUI). Mirabegonon in combination with testosterone and estradiol proved to stabilize the production of Type 3 and 4 collagens already at early observation stages in the OAB group, while Quercetin in combination with testosterone and estradiol proved to be more effective when administered at the late observation stage both in OAB and SUI models. Along with the stabilizing effect on formation of collagens and elastic fibers, Quercetin demonstrated the most pronounced antisclerotic effect (p= <0.05) compared to other medicines. In terms of efficacy as OAB treatment, the study medicines can be placed in the following sequence starting from the lowest: Spasmex ’! Mirabegon ’! Quercetin, keeping in mind that the best results were obtained by combinations thereof with testosterone and estradiol.
29. AL.TSERTSVADZE, L.EBRALIDZE, I.MATCHUTADZE, D.BERASHVILI, A.BAKURIDZE
DETERMINATION ELEMENTAL AND MINERAL COMPOSITION OF KOLKHETI
SPHAGNUM PEAT PELOIDS
Department of Pharmaceutical Technology, Tbilisi State Medical University, Georgia;
Fatih University / BINATAM (Bio-Nano-Technology R&D Center), Istanbul, Turkey
J. Experimental and Clinical Medicine, 2018, 2, pp.144-147
Peat peloids contains wide range of organic and inorganic substances that characterized with high biological activity. In order to estimate the possibility of using peat peloids in pharmaceutical or cosmetic practice, it is also important to define micro and macroelectric properties. Macro and microelements are important for the body’s homeostasis. The objective of the research was to study elemental and mineral spectrum of Kolkheti sphagnum peat peloids (Anaklia, Chirukhi). X-ray fluorescence spectrometer (XRF-EDX; 3600B) and X-ray diffraction spectrometer (Rigaku SmartLab – High Resolution XRD&Drone-3) methods were used for elemental and mineral analysis of the objects respectively.
30. K.MAZMISHVILI, N.KIKODZE, I.PANTSULAIA, M.IOBADZE, N.JANIKASHVILI, T.CHIKOVANI
ALTERED NEUTROPHIL/ LYMPHOCYTE AND PLATELET/LYMPHOCYTE RATIOS IN THE PERIPHERAL BLOOD OF PATIENTS WITH LIVER CANCER
Tbilisi State Medical University, Dept. of Immunology; TSMU V.Bakhutashvili Institute
of Medical Biotechnology, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2018, 2, pp.147-152
The pro-inflammatory effects of systemic inflammatory response have been linked with various cancers including liver cancer. The cancer-generated inflammatory response results in the upregulation inflammatory mediators, causing an increased propensity for malignancy through inhibiting apoptosis, promoting angiogenesis, and damaging DNA.
Among current systemic inflammatory markers neitrophile/lymphocyte ratio (NLR) and Platelet/lymphocyte ratio have been actively investigated.
The molecular mechanisms through which the NLR and PLR are associated with poor liver cancer outcomes remain unknown, nevertheless several hypotheses are proposed in this regard. Neutrophilia, which is responsible for inflammation, inhibits the activity of immune cells and positively associated with the progression of angiogenesis. Similarly, increased platelet frequency is associated elevated secretions of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF), which are major players in angiogenesis, cell proliferation and tumour metastasis.
Recent studies demonstrate, that the extensive lymphocyte infiltration in tumours has been related to a better prognosis in patients with cancer, however the percentages of the lymphocyte populations are diminished in patients with a higher NLR or PLR; The focus of this review is to explore and further discuss the roles of NLR and PLR, as prognostic values, in liver cancer.