J.EXPERIMENTAL & CLINICAL MEDICINE
2016, ¹ 4
THE LOST ART OF HEALING
Shota Rustaveli State University, Batumi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.8-11
Being a doctor is as science as well art. Further, introduction of modern technology of laboratory and instrument caused the crisis of the practical medicine, which is expressed in alienation of the doctor and the patient. The medicine has lost the spirituality, those principles which had been established between the doctor and the patient during the centuries. Coupling of science and doctor art is the prerequisite of the great success. Charming treatment toward the patients worth praising in terms of moral point of view, further it is very pragmatic- it is effective means of diagnostic and treatment.
2. Î.ABRAHAMOVYCH1, U.ABRAHAMOVYCH1, S.GUTA1, O.SYNENKYI2, L.TSYHANYK1
STATE OF THE AUTONOMIC NERVOUS SYSTEM IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS
1Danylo Halytsky Lviv National Medical University,
2Lviv Regional Clinical Hospital, Ukraina
J. Experimental and Clinical Medicine, 2016, 4, pp. 11-17
The article presents the results of assessment of the autonomic nervous system in 83 patients (65 women and 18 men aged 17-74) with systemic lupus erythematosus. The state of the autonomic nervous system, estimated on the basis of a careful analysis of questionnaire by A.Wayne about complaints, clinical examination using the signs of A. Soloviev and the results of functional and dynamic tests, allowed to state that all the patients with a systemic lupus erythematosus had its disbalance with a predominance of a sympathetic level activity over the parasympathetic, which has to be taken into account when treating this category of patients.
3. T.T. GABUNIA, S.D. TURABELIDZE, N.N. GOGEBASHVILI, N.V. KIPIANI, T.V. SANIKIDZE
IMPACT OF LASER TREATMENT ON THE RHEOLOGICAL PROPERTIES OF RED BLOOD CELLS IN PATIENTS WITH PERIODONTITIS
Davit Aghmashenebely University of Georgia,
Iv. Beritashvili Center of Experimental Biomedicine, Tbilisi
J. Experimental and Clinical Medicine, 2016, 4, pp.17-23
The purpose of our study was establishment some aspects of laser treatment effects in patients with periodontitis, in particular, its impact on the rheological properties of red blood cells (RBCs).
We examined peripheral blood samples from patients with middle severity periodontitis before and after laser treatment. Laser treatment was performed by apparatus “OPTODAN”–Alstom-01 during 7 days. RBCs membrane proteins have been extracted from human heparinized blood and their mobility was studied by electrophoresis method.
In RBCs membranes of patients suffering from middle severity periodontitis low molecular weight (20-45kDa) and high molecular weight (55, 97, 200, 116 kDa) proteins content reduced in comparison to those in healthy individuals. The electrophoretic mobility of RBCs membrane proteins in patients with middle severity periodontitis reduced by 10% in comparison to those in healthy individuals. After 7 days laser treatment of the severity periodontitis patients the electrophoretic mobility of RBCs membrane proteins did not change compared to initial data, while protein content with a molecular weight of 29, 34, 45 kDa increased up to the control level, and the protein fraction with molecular weight of 24kDa significant increase in compared to the control level. That indicates restoration of the actin, tropomyosin and Band 4.9 protein complex structure in the RBCs membrane cytoskeleton, which promotes increase their deformability and normalization of the blood flow in the microvessels.
Thus it can be concluded that the treatment of middle severity periodontitis by laser contributes to the normalization blood circulation, improvement of patients’ in oral cavity tissues tropism and remission of periodontal disease. At the same time it should be noted negative effect of laser on the RBC antigen CD59 (MIRL protein), that may induce decrease RBCs stability, following hypoxia of periodontal tissues and complications of the periodontitis.
4. L. JANASHVILI, O. CHOKOSHVILI, T. GABRICHIDZE, E. PERADZE
ASSESSMENT OF KNOWLEDGE, INFECTION CONTROL AND RISK OF BLOODBORNE INFECTIONS IN THE BEAUTY AND HAIRDRESSING SALONS CUSTOMERS IN THE COUNTRY OF GEORGIA, AN ANONYMOUS ONLINE SURVEY
Georgian Association for Professionals in Infection Control and Epidemiology
J. Experimental and Clinical Medicine, 2016, 4, pp.23-28
BACKGROUND. HIV, viral hepatitis and other bloodborne infections are the overgrowing public health challenges for modern world. Medical and hygienic manipulations conducted in inadequate infection control surroundings characterize with high risk of transmission of bloodborne infections among humans. A concentrated HIV epidemic, high prevalence of viral hepatitis and unsatisfactory infection control in medical and nonmedical settings is current epidemiological condition in the country of Georgia. We conducted an anonymous online survey to identify current knowledge, infection control practice and the risk of bloodborne infections in the beauty and hairdressing salons customers in our country.
METHODS. We used Google’s forms to create an anonymous online survey questionnaire with 11 questions to collect ammoniums data. The electronic questionnaire was populated using social network. Data was collected for the period of a month from middle of April 2016. Data was exported from Google forms to excel and was analyzed using R statistical language using R 3.2.3. We used descriptive statistics for distribution analysis and Pearson’s chi-squared test (P<0.05) was used to test statistical significance of association.
5. M. KHOTCHAVA, T. JOKHTABERIDZE, I. SHALAMBERIDZE, G. KHOKRISHVILI,
L. NEBIERIDZE, R. KOBAKHIDZE, M. MAISURADZE
ACRODERMATITIS ENTEROPATHICA AS RARELY DIAGNOSED DISEASE
Tbilisi State Medical University, Ltd Tbilisi, Children’s Infectious Diseases
Clinical Hospital, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.29-32
Acrodermatitis enteropathica – classically represents inherited zink metabolism disturbance. The disease is characterized by diarrhea, perioral and acral dermatitis, behavior changes, weight loss, anorexia, growth retardation, skin changes similar to atopic dermatitis. Skin impairment is typical around anatomical structures as mouth, eyes, anus, as well as on the distal parts of the extremities. Ulceration and erosions of the skin are typical in Acrodermatitis enteropathica. Secondary infections as C. albicans and S. aureus are also typical.
6. Z. NAKUDASHVILI, I. MGHEBRISHVILI, M. ENUKIDZE,
M. MACHAVARIANI, T. SANIKIDZE
PROTEIN P53 – AS PROGNOSTIC CRITERIA OF DENTURE INDUCED APOPTOSIS OF PARADONT TISSUE
Tbilisi State Medical University, Vl. Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.33-36
The aim of the study – establishment of denture induced alteration of apoptosis marker – p53. 60 patient with denture was investigated (1st and 2nd groups – 30 patient with denture on the plastic and metal basis, who used specific hygienic liquid for denture cleaning and swilling of oral cavity (1a, 2a) and not (1b, 2b); The 3rd control group – 30 volunteers with healthy oral cavity. Alterations of protein p53 content in patients’ saliva were studied by immune-enzymatic test-system «Cusabio» (before denturing; 3 days and 1 month after denturing).
Result of the study testify, that in patients with metal basis denture process is less; employing of specific hygienic liquid for denture cleaning and swilling of oral cavity decreases intensity of inflammation and therefore apoptosis.
Reviled results give us possibility to recommend saliva p53 diagnostic-prognostic marker of severity of inflammatory processes in oral cavity, develop by the course of denture induced alteration damages and also for score of effectiveness different prophylactic- treatment medicines and procedures.
7. E. BURKADZE, N. MITSKEVICH, N. CHIKHLADeZE, M. MELIKISHVILI,
N. PITSKHELAURI, B. TAYLOR
THE USE OF ANIMAL IN BIO-MEDICAL RESEARCH: ETHICAL ASPECTS
Iv. Javakhishvili Tbilisi State University, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.36-40
Use of animals for scientific discoveries counts a history of more than 2000 years. First normative act on animal care and use was issued only in 19th century, which was a first result of long anti-vivisection attitude. In 1873 US Congress passed a low of twenty-eight hours, serving as a basis for British Parliament to issue a first regulatory act on animal use in experiments.
Protection of ethical aspects of animal use in bio-medical research requires utilization of internationally approved regulations and guidelines. There are no ethical-legal regulations of animal use in bio-medical research in Georgia, neither on institutional nor on governmental level. Thus, familiarization with the US and European experience is of great importance for Georgia in development of ethical-legal regulations and harmonization with international standards.
8. SH. VASHADZE
Shota Rustaveli State University, Batumi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.40-42
250 patients with cerebral blood flow disorder were tested at Adjarian Oncocenter. Among them 114 female and 86 male. Patients were grouped according to scale of stroke , developed by National Health Institute. Plasminogen Activator (t-PA) was most effective during acute stroke. Hospitalization of patient should not be oriented only for treatment of stroke but also about identifying Risk factors. Multidisciplinary (doctors, physiotherapists, occupational therapist, psychologists, specialized nurses and social workers) group should be involved in rehabilitation process, which is the key for managing stroke patients.
9. Z. NAKUDASHVILI, I. MGEBRISHVILI, M. PAPAVA, M. ENUKIDZE, M. MACHAVARIANI,
T. MCHEDLISHVILI, T. SANIKIDZE
DENTURE INDUCED ALTERATIONS OF IMMUNE AND REDOX BALANCE OF PATIENTS
Tbilisi State Medical University, Vl. Bakhutashvili Institute of Medical Biotechnology, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.43-47
The aim of the study – establishment of denture induced alterations of immune and redox balance in patients blood. 60 patient with denture was investigated: 1st group – 30 patient with denture on the plastic basis, who used specific hygienic liquid for denture cleaning and swilling of oral cavity (1a) and not (1b); 2nd group – 30 patients with denture with metal basis, who used specific hygienic liquid for denture cleaning and swilling of oral cavity (2a) and not (2b). The 3rd control group – 30 volunteers with healthy oral cavity.
Result of the study indicates the different intensity of disorders of immune and redox balance odpatients blood, depending on the type of denture, It was testified, that in patients with metal basis denture inflammatory process is lessintensive; employing of specific hygienic liquid for denture cleaning and swilling of oral cavity decreases intensity of inflammation.
10. S.TURABELIDZE, T.GABUNIA, M.PAPAVA, N.KIPIANI, G.ORMOTSADZE,
M.MACHAVARIANI, M.ENUKIDZE, T.MCHEDLISHVILI, N.GOGEBASHVILI, T.SANIKIDZE
ANTHRANILIC ACID AS A MARKER OF PERIODONTITIS IN DIABETIC PATIENTS
Tbilisi State Medical University, Vl. Bakhutashvili Institute of Medical Biotechnology;
Davit Aghmashenebely University of Georgia, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.48-52
The purpose of the study was establishment of the correlation between anthranilic acid concentration in patients’ saliva and patients’ oral cavity parameters representing severity of the paradontitis during diabet mellitus. It was concluded that:
- Markers of inflammation, TNFα, IL10 and IL2, in the blood can determine the severity of periodontitis only and are not reveal a causal link to severity of the diabetes.
- Anthranilic acid (its content in saliva), can not be used as a marker of the severity of periodontitis, but clearly reflects the existence of periodontitis.
- Increase inanthranilicasid acid can be viewed as a trigeringmechanism between two separate marker (TNFα and IL2 increase) in pathogeneticchain of paradontitis development.
11. K. UKLEBA, I. PAVLENISHVILI, D. ZURABASHVILI
PERINATAL HYPOXIC – ISHEMIC DAMAGE OF CENTRAL NERVOUZ SYSTEM AND COGNITIVE DEVELOPMENT OF TEENAGERS
Tbilisi State Medical University, Institute of Post-Graduate Medical Education and Coherent Professional Development, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.52-56
The purpose of research was to study the negative affect of perinatal hypoxic – ischemic damage of central nervous system on teenagers cognitive development.
Random research has revealed that among the main group (teenagers with perinatal pathologies) and the control group representative the relative risk of low IQ is approximately 2,26; while relative risk of memory deficiency is 1.34. The research also revealed that the relative risk of attention deficiency span among the main and control group representative is 1.36.
12. G. GURGENIDZE, D. LABARTKAVA, N. URUSHADZE
AN ASSESSMENT ALGORITHM FOR CLINICAL EVALUATION OF THE STEROID-SENSITIVITY IN PATIENTS WITH BRONCHIAL ASTHMA
V. Bakhutashvili Institute of Medical Biotechnology of Tbilisi State Medical University,
J. Experimental and Clinical Medicine, 2016, 4, pp.57-59
On the basis of clinical assessment and laboratory (immunology) testing of 96 asthmatic patients and 42 healthy volunteers, with further evaluation of correlation and statistical significance of above-mentioned data, it have been elaborated strictly objective clinical criteria (diagnostic algorithm) for determination of steroid-sensitivity in this population. These clinical criteria, including spirometric values, will be helpful for prediction of the responsiveness of each individual patient to the planned steroid treatment with respective evaluation of their possible risks and expected benefits.
13. M.GONGADZE, N.GOGIA, TS. ATAMASHVILI, A.SEPASHVILI, M.IOBADZE
THE KEY MOLECULES IN DIABETES-INDUCED PREGNANCY LOSS
TSMU V.Bakhutashvili Institute of Medical Biotechnology;
P.Shotadze Tbilisi Medical Academy, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.59-65
Perinatal mortality is one of the main complications of diabetic pregnancy. Mechanisms underlying diabetes-induced such outcome we have investigated in our study. The results of our investigations suggest that IL-10 and TNFα play a central role in the pathogenesis of diabetes- induced pregnancy loss. TNFα act as a mediator of diabetes-induced embryotoxic stimuli leading to the death of peri-implantation stage embryos or resorbtion formation, and IL-10 as a suppressor of diabetes-induced abortion in this stage. Diabetes induces reduction of IL-10 production, sustained increased TNFa expression in uterine cells upregulates following Nitrogen Free Radicals synthesis, resulting in increase of apoptosis process intensity and decrease cell proliferation which is represented in low uterine weight. Also, early stage pregnancy loss could be a result of dysregulation and then dysfunction of molecules playing key roles in the acquisition of receptivity by the uterus and successful implantation such as Matrix Metalloproteinase 9 and LIF.
Taken together, these results suggest that IL-10, TNF-alpha, LIF, MMP-9 and NO coordinated expression and function are critical for a viable pregnancy.
14. N. KANTARIA, G. SIMONIA
SALT-SENSITIVITIVITY AMONG THE PEDIGREES OF THE PATIENTS WITH ESSENTIAL HYPERTENSION IN GEORGIA
Department of Internal Medicine, Tbilisi State Medical University, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.65-68
The aim of our study was to investigate the salt-sensitivity in Georgian pedigrees of patients with salt-sensitive essential hypertension. The objectives was typing the patients with essential hypertension and their pedigrees by salt-sensitivity test into salt-sensitive and salt-resistant subjects in order to evaluate the spread of salt-sensitivity among the families. 89 middle aged (30-72) ethnically Georgians were enrolled into the study. Among them 42 were patients with essential hypertension stage 1 (JNC 7), and 47 their family members. The salt-sensitivity was detected in 62% (26) of patients and 57.5 % (27) of pedigrees.
The majority of salt-sensitive pedigrees 85.2% were of salt-sensitive patients family members that support the idea that genetic factor might play the key role in development of salt-sensitivity. Salt-sensitivity test can be used in patients with essential hypertension in order to define the treatment plan in accordance with pathogenesis. Also, Investigation of family members of these patients will contribute to primary prevention of hypertension among pedigrees.
15. O.O. SYZON
PSORIATIC ARTHROPATHY: DEBATING ISSUES
Danylo Halytsky National Medical University of Lvov
Department of Dermatology and Venereology, Ukraine
J. Experimental and Clinical Medicine, 2016, 4, pp.68-79
Aim. The aim of this paper is to harmonise the representation of principal problematic issues of psoriatic arthropathy (PA) development and to devise the classification, true clinical and diagnostic features and algorithm of its differential diagnostics with further prescription of pathogenetic treatment based on the analysis of the literary sources and the author’s own observations.
Materials and methods. The analysis of literary sources and the author’s own observations, made during 2011-2016, of 187 patients with PA, who underwent differential clinical laboratory and additional instrumental examination in hospital environment (X-ray diagnostic, ultrasound investigation, densitometry, MRT), has been conducted.
Results and discussion. This paper presents the results of such analysis and offers a novel vision of certain disputable aspects of PA pathogenesis, clinical picture, diagnosis, and treatment. The paper describes PA clinical signs and diagnosis criteria developed as an additional feature to ensure the uniformity of diagnosis, and facilitate differential diagnostics and prescription of the appropriate treatment.
Conclusions. Today, from the perspective of generalized enthesopathy, PA should be viewed as an autoimmune and genetically induced disease, hence the cases of clinically detected arthralgia not backed up by X-ray evidence are present. When performing differential PA diagnostics, it is necessary to remember that in most PA patients joints damage occurs concurrently with psoriasis and is congruent with nail pathologies. Articular syndrome manifests itself as asymmetric peripheral or central mono- or oligoarthritis with the prevailing damage of distal interphalangeal joints of the hands. In PA cases X-ray examination reveals the prevalence of osteodestructive (osteolysis, ankylosis) and osteoproliferative (hyperostosis, periostitis) pathological manifestations over osteoporosis.
16. E. ARAKHAMIA, T. CHIKOVANI, L. JANASHVILI, N. BARNABISHVILI, L. GOGICHISHVILI
COMPARISON OF RESULTS OF ELISA AND ICT TESTS IN DIAGNOSIS
OF CYSTIC ECHINOCOCCOSIS
S. Virsaladze Institute of Medical Parasitology and Tropical Medicine, Tbilisi, Georgia
J. Experimental and Clinical Medicine, 2016, 4, pp.82-82
Echinococcosis in humans is a disease caused by the larvae of Echinococcus granulosus (E. granulosus) and Echinococcus multilocularis (E. multilocularis). The diseases caused by these parasites represent a substantial burden on the human population. Present estimates suggest that cystic hydatid disease, caused by Echinococcus granulosus, results in the loss of 1–3 million disability-adjusted life years per annum. The annual cost of treating cases and economic losses to the livestock industry probably amounts to US$ 2 billion.
Ultrasonography imaging is the technique of choice for the diagnosis of both cystic echinococcosis and alveolar echinococcosis. Specific antibodies are detected by different serological tests and can support diagnosis. Serological tests are valuable, especially in the clarification of unexplained clinical findings and imaging methods. The purpose of this study is to investigate the comparison of results of an immunochromatographic test (ICT) specific for E. granulosus antibodies and enzyme-linked immunosorbent assay (ELISA) in the diagnosis of echinococcosis.