J. EXPERIMENTAL  &  CLINICAL  MEDICINE

2016, ¹ 2

 

 

 

1. NIKOLAISHVILI M., CHICHINADZE K., JIKIA G., MUSELIANI  T.  

ANIMAL BEHAVIOR, NEUROCHEMICAL PROFILE IN AGGRESSIVE AND

NON-AGGRESSIVE RATS

Iv. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.8-11

 

Thus it should be considered the main component participated in aggressive behavior. Due to the above mentioned the role of serotonin in aggressive behavior should not be lost. Hence based on the literature as well as on our earlier data, right the ratio of serotonin concentration to noradrenalin represents the organism aggression level and hierarchy rank.

Thus noradrenalin could be the stimulating factor for aggressive behavior , although we should note that specific aggressive agent is not discovered yet. And it seems that the component carrying only this function is not produced in organism.

There are various forms of aggressive behavior: predatory aggressiveness, aggression among males, aggression caused by fear and aggression caused by irritation etc. The literature knows dominnat aggressive as well as  subordinate less aggressive animals’ and humans’ behavior, also bio- and neurochemical properties of their bodies [6]. It is also known that aggressive behavior has a role in the formation of dominant hierarchy, at the same time memory and learning skills should help these animals to gain higher hierarchic rank. Thus, in order to detalize the above mentioned information we have decided to study rats, that have not got social relationship experience in the group. That is they are relatively young immature and selected them by Yumatov method as more aggressive and less aggressive rats and studied them passive avoidance method designed by Buresh in order to determine how the aggressive rats differ from non-aggressive ones and whether they have skills characterized for the present hierarchy in even immature age: learning skills, rate of aggressiveness, etc. [1,4]. 

 

2. JAPARIDZE N., ZARNADZE I., KHAZARADZE K., ZARNADZE SH.,  BARAMIDZE L.,

LOMTADZE L., KAJRISHVILI M.

EVALUATION OF CHILD CARE SYSTEM SITUATION IN GEORGIA

TSMU Public Health, Management, Policy and Economy Department, Tbilisi, Georgia;

Ministry of Labour, Health and Social Affairs of Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.12-14

 

The existence of strong system is essential for successful functioning, which includes different directions, such as: health, social conditions improvement, child protection from violence, poverty, realization of their rights and the most important – upbringing in the family environment, adequate education and social (needs based) service provision.

The child care system reform is active in Georgia during the last years, but children are still in big residential institutions (children with disabilities), the one of the main reasons is delays in development of the home care service financed by the state.

In this regard it is important to develop small group homes network and further support of day care centers services and accordingly, opening of the new day care centers and advance other alternative care services.

 

3. NINO MIRZIKASHVILI, LOUISE-ANNE MCNUTT

ADOLESCENT HEALTH SERVICES IN GEORGIA

Tbilisi State University, Faculty of Medicine, Tbilisi, Georgia;

Epidemiology and Biostatistics School of Public Health; Institute for Health and the Environment; University at Albany, State University of New York, USA

J. Experimental and Clinical Medicine, 2016, 1, pp.14-21

 

Objectives: The goal of the study was to evaluate adolescents’ health services and elaborate recommendations in order to improve adolescents’ health services in Georgia.

Methods: In order to evaluate adolescents’ health services in Georgia, Youth Health Better Practice Framework has been used. Additionally the quantitative survey was conducted among 11-19 years old adolescents. Overall 1000 young people were interviewed across Georgia using a standard questionnaire in 2014.The survey sampling methodology was multi-stage probability sampling. All information were analyzed in SPSS 21 version.

Results: Little research has been conducted among young people in Georgia. There are no programs which specifically target young people. According to the HUES 2014 financial barriers to access have declined since 2010, mainly for outpatient visits. However adolescents still have a problem to access medical facility. Medical personal are not trained especially in adolescent health issue. Generally, the staff have not been provided with training, supervision and support to maintain the knowledge and skills required for working with young people.

Conclusion: Young people should have easy access to medical facility. It is recommended to develop national quality standards and monitoring systems in order to better respond to the health and development needs and opportunities of adolescents. Medical facility should offer comprehensive, age appropriate, youth-friendly health services with appropriate staff trained to treat youth respectfully and confidentially.

 

4. NIKOLAISHVILI M., CHICHINADZE K., JIKIA G., MUSELIANI T.

NEUROCHEMICAL CORRELATES AND ENERGY SHIFTS OF AGGRESSIVE AND

NON-AGGRESSIVE ANIMALS

Iv. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.21-23

 

Determination of workability and endurance in more aggressive and less aggressive animals on early stages of ontogenesis Due to the above mentioned the role of serotonin in aggressive behavior should not be lost. Hence based on the literature as well as on our earlier data, right the ratio of serotonin concentration to noradrenalin represents the organism aggression level and hierarchy rank. These changes definitely reflect on animals’ memory and learnability. According to the researches the activation of the energy shifts in aggressive animals effects the fast formation of new protein and all this denotes the high quality of the consolidation and memory of aggressive animal.

 

5. E. KVARATSKHELIA1,2, N. DABRUNDASHVILI1, E. MAISURADZE1, M. GAGUA1,

M. KAMKAMIDZE1, E. ABZIANIDZE2

A LINK  BETWEEN DNA METHYLATION AND HISTONE MODIFICATIONS IN T-CELLS FROM PATIENTS WITH CYSTIC FIBROSIS

Tbilisi State Medical University, Vl.Bakhutashvili Institute of Medical Biotechnology1,

Department Of Molecular and Medical Genetics2, Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.24-27

 

Cystic fibrosis (CF), the most common autosomal recessive  disorder is caused by mutations in the Cystic Fibrosis Ttransmembrane Conductance regulator (CFTR) gene. T-lymphocytes from patients with CF are characterized by reduced expression of anti-inflammatory cytokines such as IL-10 and elevated levels of pro-inflammatory cytokines (IL-8). Although mechanisms responsible for cytokine  dysregulation is not entirely clear epigenetic mechanisms such as DNA Methylation and histone modifications might contribute. In the present study we compared levels of histone H3K4 methyltransferase and DNMT3b between patients with CF and healthy controls. 

The study was approved by the Biomedical Research Ethic Committee of the Tbilisi State Medical University and informed consent was obtained from all participants. Peripheral blood (5-7 ml) was obtained by venipuncture from CF and healthy subjects. Levels of histone H3K4 methyltransferase and DNMT3b in nuclear extracts of CD4+ T-cells were measured using Activity Quantification Assay Kits (abcam) according to the manufacturer protocol. 

We found that the level of  DNMT 3b was significantly higher in patients with CF than in healthy controls, while histone H3K4 methylatransferaselevels  were significantly reduced in CF CD4+ T-cells compared with healthy control CD4+ T-cells. Correlation analysis showed that DNMT 3b levels was negatively correlated with the expression level of histone H3K4  methylatransferase. 

Our results suggest that the overexpression of DNMT3b and  reduced histone H3K4  methylatransferase levels may contribute to aberrant gene expression in CD4+ T-cells of patients with CF. Epigenetic markers  might be useful  for clinician estimate the activity of Cystic Fibrosis  able of detecting high-risk patients as well as  for the efficient clinical management of these patients.  

 

6. MURJIKNELI K.G., GIVIASHVILI V.A., ZARNADZE I., ZARNADZE SH., LOMTADZE L.,

KITOVANI D., KAJRISHVILI M.

EVALUTIONS OF THE AVERAGE AND COLLECTION DOES OF PROFESSIONNAL IRRADIATION IN THE MEDICAL PERSONNEL

Tbilisi State Medical University

J. Experimental and Clinical Medicine, 2016, 1, pp.27-29

 

Average and collection does of irradiation of medical personnel exposed to professional irradiation have been evaluated. Parameters of moral risk produced by additional malignant tumors, genetic defects have been calculated as well as the number of remot possible stochastic effects in the professional group under study. The results obtained show no real danger of an increase in the number of stochastic effects.

 

7. CHICHINADZE K., NIKOLAISHVILI M., JIKIA G., MUSELIANI T.

WORKABILITY, ENDURANCE AND ENERGY SHIFTS IN CONSOLIDATION AND MEMORY IN AGGRESSIVE AND NON-AGGRESSIVE ANIMALS

Iv. Beritashvili Center of Experimental Biomedicine, Tbilisi, Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.30-33

 

Thus, based on the above we can conclude, that the animals which have not formed their hierarchical status yet and are only 60 days old and weigh 75 gramms (immature offsprins), in other words, already in offspring, until the social hierarchy is formed, the difference between both aggressive animals from non-aggressive ones by their workability and endurance is clearly noticeable. Intensified energy change in aggressive animals causes increase of workability, these changes definitely reflect on animals’ memory and learnability. According to the research the activation of the energy shifts in aggressive animals effects the fast formation of new protein and all this denotes the high quality of the consolidation and memory of aggressive animals.  

 

 

8. GURGENIDZE G., LABARTKAVA D., URUSHADZE N.

PRELIMINARY ASSESSMENT OF EXPECTED RESPONSIVENESS TO THE TREATMENT IN PATIENTS WITH BRONCHIAL ASTHMA ACCORDING TO THE RESULTS OF DETERMINATION OF PERIPHERIAL BLOOD BASOPHILS STEROID-SENSITIVITY

Vl. Bakhutashvili Institute of Medical Biotechnology of Tbilisi State Medical University, Georgia

J. Experimental and Clinical Medicine, 2016, 1, pp.34-37

 

In vitro steroid-sensitivity of peripherial blood basophils, obtained from GCS-responsive and unresponsive asthmatic patients and healthy adult donors, subsequently incubated with different concentrations of dexamethasone (1 nM – 1 mM), has been evaluated using the highly reproducible staining method with Alcian Blue. According to the results of study,  basophils of GCS-unresponsive asthmatic patients were statistically significantly much more resistant (more viable cells) to the cytolitic action of physiologic, as well as pharmacologic in vitro concentrations of dexamethasone, than basophils, obtained from GCS-responsive patients and control subjects.