EXPERIMENTAL & CLINICAL MEDICINE
2008, ¹ 5
1. Gogichadze G., Beniashvili D., Gogichadze T.
fusogenic effects OF Lymphoid TYPE cells at early stages of Moloney virus-induced Lympholeukemia
J. Experimental and Clinical Medicine, 2008, 5, pp. 9-14.
Tbilisi State Medical University, Department of Microbiology and Immunology, Georgia
The karyogamy theory of carcinogenesis has been getting more popular paradigm in cancer research for the last years. This hypothesis is based on the fusion of two normal cells and the following development of precancerous, and finally - tumour cells. Hence, it would be very interesting to scrutinize the early morphological changes in lymphoid cells infected by the virus of Moloney. For a visual study of the infected cells, light-optical and electronic microscopy was applied. Materials were examined in dynamics. Earlier than the first hours after contamination (2 hours and less) the interactions and adhesions of lymphoid cells as well as cells fusion were observed, expressed in the formation of two- and three-nuclear cellular structures. Moreover, the majority of the investigated cells were homokaryons. Thus, early morphological changes in lymphoid cells after contamination with the virus of Moloney suggest that the fusion of nuclei or karyogamy contributes to the development of a cancer cell.
2. T.MCHEDLURI, G.KARCHAVA, E.PETRIASHVILI, T.SOLOSHVILI, L.VASADZE,
T. MUSELIANI, K.NADAREISHVILI
The Effect of power-Frequency Electromagnetic Field on the development OF PASSIVE AVOIDANCE REACTION IN ADULT Rats
J. Experimental and Clinical Medicine, 2008, 5, pp. 14-16.
Research Center of Radiation Biology and Ecological Radiology, Tbilisi
The effect of power-frequency electromagnetic field (50Hz/1.5mT) provokes significant changes in the formation and storing of passive avoidance reaction in adult rats. In particular, the exposed rats show depressed character, and the development of passive avoidance reaction is significantly decreased in them.
3. MAMEDHASANOV R., VELIKHANLY R.
A COMPARATIVE ANALYSIS OF THE CONCENTRATION OF ANP (1-98) AND ADRENAL GLAND HORMONES IN THE BLOOD OF AH PATIENTS (BEFORE AND AFTER TREATMENT) AND HEALTHY PEOPLE DURING A GEOMAGNETIC STORM
J. Experimental and Clinical Medicine, 2008, 5, pp. 16-22.
Azerbaijan Medical University, Baku
The objective of the research is to study a clinical significance of the level of ANP and adrenal gland hormones in the blood plasma of a patient with AH with exacerbated condition (as a result of a geomagnetic storm). Three groups were examined: 30 patients in the main group, 10 practically healthy men in order to conduct a comparative analysis in the second group, and 10 patients with AH - in the third group. The results of the researches were tested in each of there groups on a day of geomagnetic storm. There was found a correlative dependence between ANP (1-98), adrenal gland hormones and arterial pressure.
Subsequently, against the background of AT that enhanced due to the geomagnetic storm, the level of ANP (1-98) and adrenal gland hormones increased. 30 patients listed in the main group underwent the treatment with prestarium, athenolol and ariphon retard.
As AT was not increased in the treated patients of the main group during the magnetic storm, all indications were reduced (p<0,001). There were found very little changes in the concentration of reduced glutathione and Malon dialdehyde in the catalase activity.
However, there was found no serious difference in the conclusions of the examinations taken in the first and second groups on the day of a geomagnetic storm. We may come to the conclusion that prestarium and athenolol, adjusting neurohumoral regulation of blood circulation, reduce the concentration of ANP and adrenal gland hormones in the blood plasma, and subsequently prevent more serious side-effects.
4. Nasirova R.
PREOPERATIVE EVALUATION OF GERIATRIC PATIENTS WITH UROLOGICAL PATHOLOGY
J. Experimental and Clinical Medicine, 2008, 5, pp. 22-26.
Department of Anesthesiology and Resuscitation, A.Aliyev Azerbaijan State Institute for Advanced Training of Physicians, Baku
Preoperative status of 900 urological patients of the age group 65-96 was investigated in comparison with 252 middle-aged patients. The research results have shown that the rate of concomitant chronic conditions increases with the age of patients. It was revealed that the concomitant heart and respiratory problems increase intensively in this group of patients. The incidence of two or more concomitant diseases in the same patient also increases with the age. The author concludes that the above-mentioned factors are the main indicators of the preoperative status of geriatric urological patients.
5. Jandieri L., Kikalishvili L., Khomeriki Ts., Pachkoria V.
Lipid Peroxidation in the Microsomal
Fraction OF Liver and Gastric Mucosa after TrunCal Vagotomy
J. Experimental and Clinical Medicine, 2008, 5, pp. 26-30.
Department of Topographic and Clinical Anatomy, Tbilisi State Medical University, Georgia
The aim of this investigation is to study disturbances of lipid peroxidation in the microsomal fraction of the liver and gastric mucosa after experimental truncal vagotomy.
Experiments have been carried out on 12 white laboratory rats. The material for investigation was taken on the 3rd, 10th, and 30th days after the vagotomy.
Investigations have shown that vagotomy leads to the destabilization of lipid peroxidation in the membranous structures of the endoplasmic reticulum. It is necessary to note that the microsomes of gastric mucosa are more susceptible to the influence of this intervention than liver microsomes.
The revealed changes of lipid peroxidation intensity show the characteristic features of the harmful factors on the microsomal lipid peroxidation system, which plays a major role in the regulation of intercellular chemical homeostasis.
6. Novruzov R.
morpho-histochemical aspects of DENSE fibrillar connectiVE TISSUE In women with prolapsE of small pelvis organs
J. Experimental and Clinical Medicine, 2008, 5, pp. 30-34.
Azerbaijan Medical University, Baku
Hospital for Reparative Treatment, Sumgait, Azerbaijan
Pathogenesis of the prolapse of small pelvis organs in women can be connected with anomalous changes in the dense fibrillar connective part composing aponeurosis, fascia and reticular layer of the derma. Aponeuroses of the oblique muscle of the anterior abdominal wall, round ligament of the uterus, sacrouterine ligament, reticular layer of the derma of the anterior abdominal wall and inguinofemoral region were microscopically analyzed in 58 women for the condition of the structure of dense fibrillar layers.
The contingent was divided into 2 groups: 1. group of comparison - 22 women without clinical signs of prolapse of the internal organs and small pelvis; 2. main clinical group with isolated and combined clinical forms of the prolapse of small pelvis organs.
Fibrosclerosis, diselastosis, reduction of microcirculation, anomalously high level of GAG, and suppression of key ferments activity can cause pathogenesis in aponeuroses and ligaments during prolapse of small pelvis organs. These changes can influence different results of surgical treatment of pelvic organs prolapse. The data demand a special attention to the condition of dense fibrillar connective structures in women with these pathologies.
7. SALAKHOVA S.
COMPARATIVE EVALUATION OF THE RESULTS OF OPEN AND LAPAROSCOPIC APPENDECTOMY FOR COMPLICATED FORMS OF ACUTE APPENDICITIS
J. Experimental and Clinical Medicine, 2008, 5, pp. 34-38.
Department of Surgical Diseases ¹1, AMU, Baku, Azerbaijan
The aim of this study was to compare the results of open and laparoscopic appendectomy for complicated and atypical appendicitis. 60 open, 61 laparoscopic and 2 video assisted appendectomies have been executed. 2 cases demanded conversion, and 1 case - relaparoscopy. An improved method for laparoscopic appendectomy for atypical locations of the appendix and complicated types of acute appendicitis has been worked out. The results of endosurgical management showed an improvement in the rate of complications and hospital stay in comparison with the control group (open surgery only).
8. Kunchulia L., Makharadze R., Imedadze A., Chikviladze T.
Standardization of rectal Tocoprol suppositories
J. Experimental and Clinical Medicine, 2008, 5, pp. 38-41.
Tbilisi State Medical University, Georgia
Apitherapy or the science of treatment with help of beekeeping products in spite of its modern name has the ancient background in traditional medicine of different nations. Propolis and its derivatrives are characterized with antimicrobial, antimycotic, antiviral, anti-inflammatory, anesthetic, antioxidative and anticancer activity. The treatment of chronic prostatitis is rather complex and consists of antibacterial and general strengthening therapy, also local treatment of the prostate. Georgian propolis is reach in biologically active compounds. For the purpose of prostatitis and prostate adenoma treatment, there has been developed Tocoprol - a combined preparation summarizing all the positive properties of propolis and a-tocopherol. Propolis stimulates adaptive reactions of human body; a-tocopherol stabilizes cell membrane and removes inflammatory processes. Lipophilic base provides an easy biological penetration of active compounds into the place of inflammation. On the basis of Tocoprol standardization, the pharmacopoeia article was created. The medicine has passed clinical approbation.
9. AMIRASLANOV A., MURADOV KH., VELIYEVA KH.
PECULIARITIES OF ELECTRON MICROSCOPY AND HISTOCHEMICAL INVESTIGATION IN DIAGNOSIS OF NON-INFILTRATIVE ENDOCRINE CELL CANCER OF THE MAMMARY GLAND
J. Experimental and Clinical Medicine, 2008, 5, pp. 41-45.
Morphological structure and hormonal status of endocrine cell breast cancer have a big role in clinical behavior and prognosis of the disease. Therefore, it is important to estimate the endocrine component as a diagnostic and prognostic criterion determining the strategy of the following treatment.
10. AHMEDOV E.
RESULTS OF TREATMENT OF PATIENTS WITH CHRONIC TEMPOROMANDIBULAR JOINT ARTHRITIS
J. Experimental and Clinical Medicine, 2008, 5, pp. 45-47.
Department of Oral and Maxillofacial Surgery, Azerbaijan Medical University, Baku
Temporomandibular joint diseases and damages are among the bone-joint system pathologies with the most complicated diagnosis. We used selective inhibitors of cyclooxsigenaze-2 - preparation Nimesulid and ultrasonic therapy with the preparation Kortospan in the complex treatment of chronic temporomandibular joint arthritis. The application of new methods in the complex pathogenetic treatment has led to the fast recovery of the patients and caused a positive clinical effect.
11. MAMEDHASANOV P., MUSTAFAEVA A.
A COMPLEX STUDY OF MORPHOCONSTITUTION INDEXES IN PATIENTS WITH DIABETES MELLITUS AND DIFFERENT TYPES OF SUBCUTANEOUS FAT DISTRIBUTION
J. Experimental and Clinical Medicine, 2008, 5, pp. 47-51.
Azerbaijan Medical University, Baku
The present article presents a complex study of morfoconstitution indexes, research of carbohydrate metabolism in patients suffering from pancreatic diabetes and in control persons with various types of subcutaneous fat distribution.
On the base of the mentioned researches, it is possible to single out and characterize a type of morphoconstitution associated with subcutaneous fat distribution peculiar to the patients suffering from pancreatic diabetes, as well as to evaluate the present type of morphoconstitution, degree and character of lipopexia, in particular - of carbohydrate metabolism. There was determined the influence of lipopexia degree and character (particularly of carbohydrate metabolism), and patient’s clinical presentation for studied persons of Azerbaijani population with the aim to reveal the frequency and features of the course of pancreatic diabetes, as well as of the other abnormalities of carbohydrate metabolism in persons with alimentary obesity.
12. NASIBOVA ESMIRA MIRZA qizi
A COMPARATIVE ANALYSIS OF THE EFFECT OF NARCOTIC ANALGESICS COMBINED WITH LIDOCAINE IN SACRAL ANESTHESIA FOR PROCTOLOGICAL PATIENTS
J. Experimental and Clinical Medicine, 2008, 5, pp. 51-55.
Clinical Medical Centre, Baku , Azerbaijan
Modern anesthesiology has to solve two important problems: I – to protec the organism from nociceptive pain impulses; II – to stabilize the autoregulatory system of the organism in order to avoid intraoperative and postoperative stress.
Sacral anesthesia is the most feasible anesthetic method for proctological patients. Sacral anesthesia is more protective than several types of general anesthesia. It blocks pain impulses and renders less harmful effect on the important systems of the organism. Nowadays, combinations of narcotic and local analgesics are widely used for sacral analgesia in all clinics.
The reason for this research was to determine the optimal combination of analgesics for sacral anesthesia: I variant – lidocaine only; II – lidocaine with narcotic analgesics (morphine, fentamine, promedol, omnopon).
The study was performed on 200 patients in the Department of surgical diseases N1 of AMU. For the study were used the following methods of examination: subjective-objective, clinic-functional and laboratory.
The investigation has shown that narcotic analgesics and lidocaine used together improve the quality of premedication, prolong the duration of anesthesia, minimally affect the vegetative nervous system. Sacral anesthesia can be used as an alternative to general anesthesia for proctological patients.
13. L. Todua, E. Mshvidobadze
Eyedrops Oxial for treatment of ”dry eye” syndrome
J. Experimental and Clinical Medicine, 2008, 5, pp. 55-57.
Department of Ophthalmpology, Tbilisis State Medical University, Georgia
“Dry eye” syndrome is the most frequent diseases seen in today`s ophthalmologic practice. It is rarely associated with systemic diseases.
Purpose: Investigation of efficiency of eyedrops Oxial (Santen).
Methods: The study was carried out on 30 patients (60 eyes).
Results and conclusions: The study shows that monotherapy of the eyedrops Oxial is quite effective lubricant remedy in cases of low and middle stage of the disease.
14. Kikalishvili L., Ramishvili M., Pachkoria V., Sulaberidze G., Kipiani E.
Morphological Changes of the Kidney after liver Ischemia-Reperfusion
J. Experimental and Clinical Medicine, 2008, 5, pp. 57-62.
Department of Topographic and Clinical Anatomy at the Tbilisi State medical University, Georgia
The experimental animals were divided into 3 groups. The I group of animals (n=3) served as control, in the II group (n=12) the hepatoduodenal ligament was occluded in normothermic conditions. In The III group (n=12) the hepatoduodenal ligament was occluded in hypothermic conditions.
After liver hypothermic Ischemia-reperfusion the histological, histochemical and ultrastructural changes in the Kidney indicate injury and reparation dynamic.
The injury of Kidney is revealing 48 hours after ischemia-reperfusion. The injury is mild 15 min after liver reperfusion. The process is progressing and the pick of the injury is revealing
48 h after liver reperfusion. The changes into the kidney are normalizing 3 month after liver reperfusion.
15. PACHKORIA V., KIKALISHVILI L., JANDIERI L., RAMISHVILI M.
THE ANALYSIS OF IMMUNOHYSTOCHEMICAL RESULTS IN LOCALIZED
J. Experimental and Clinical Medicine, 2008, 5, pp. 62-65.
Department of Tophografic & Clinical Anatomy, Tbilisi State Medical University;
The analysis of immunohystochemical results showed that in the patients of the localised carcinoma, the HER-2/Neu antigen was negative in 47 patients (69.1%); 9 patients had (13,2%) 1+ positivity; 2+ positivity in 7 patients (10,3%) and 3+ positivity (7,4%) in 5 patients.
16. T.Mchedluri, M.Nikolaishvili, M.Svanidze, T.Museliani,
Influence of the electromagnetic radiation on behavior
white and black rat
J. Experimental and Clinical Medicine, 2008, 5, pp. 65-67.
Scientific Center of Radiobiology and Radiation Ecology , Tbilisi, Georgia
The Electromagnetic radiation (EMI) acts on shaping and conservation passive avoidance reaction in rats. As follows, EMI causes beside animal of the difficulty and suppresses already worked out reaction passive avoidance.