SHERVASHIDZE M., BARBAKADZE K., AKHVLEDIANI I.

CHANGES OF CELLULAR AND HUMORAL INDEXES OF THE IMMUNE SYSTEM IN CHILDREN OFTEN SUFFERING FROM NON-SPECIFIC DISEASES OF THE RESPIRATORY SYSTEM

Experimental and Clinical Medicine, 2006, 2, pp.9-12.

 

I.Javakhishvili Tbilisi State University, Chair of Pediatrics,Tbilisi, Georgia

 

Infections of the upper and lower respiratory tract are usually mild and self-limited. Owing to their frequency recurrent lower and upper respiratory tract infections in children and adults constitute a major global health problem. The goal of our study was to observe 30 children with recurrent respiratory tract infection (17 boys and 13 girls). They were treated with RIBOMUNYL. The control group consisted of 15 virtually healthy children (10 boys and 5 girls). The age of the children ranged between 8 months and 6 years. The study was conducted by the simple randomization method. Clinical and laboratory examinations were evaluated before and after the treatment. The results of our study are given in the table. The concentration of INF-ã was rather low in the study group in comparison with the control group before the beginning of the treatment that indicates insufficient stimulation of T-lymphocytes in children with recurrent infection. INF-ã level significantly increased ! after the treatment (statistically significantly P-0.05). Neopterin level unlike INF-ã in the study group was higher than in the control group before and after the treatment. This fact can be explained so – biologically active substance level remains high in the presence of recurrent infections. Ribomunyl treatment significantly increased the level of neopterin. The efficacy of the product is primarily retested by a significant reduction in the number of recurrent infection episodes and the use of antibacterials. A combined immunostimulating effect of Ribomunyl makes it possible to use the preparation for creating a long-term postvaccinal protection of the child.

 

 

MELIKADZE E., CHICHINADZE N., SUMBADZE TS.

Impact of Phenol Intoxication on Concentration of Gonadotrope and Sex Hormones IN Experiment

Experimental and Clinical Medicine, 2006, 2, pp.12-14.

 

A.Natishvili Institute of Experimental Morphology Georgian AS, Tbilisi, Georgia

 

The target of our research is to define alterations of gonadotrope and sex hormones during intoxication with phenol vapor.

The research was held on 20 white grown - up male rats of mass about 200-250 gr. During 15 days each animal daily day experienced phenol vapor inhalations in a special covered chamber.

The animals were researched after decapitation on the 16th and 45th days of the experiment. The concentration of gonadotrope and sex hormones was defined in the blood serum. The data received during the research of the control rats were considered to be the initial value. During the experiment the alteration of sex steroids was expressed, namely the concentration of estradiol increased up to 16.0 ng/ml on the 16th day, and on the 45th day made 8.0 ng/ml (N – 6.2 ng/ml). The concentration of estrone increased up to 72.0 ng/ml on the 16th day and decreased to 38.0 ng/ml (N – 48.0 ng/ml) by the 45th day, a significant alteration of testosterone was expressed, on the 16th day it decreased to 0.5 ng/ml and on the 45th day it again made 0.5 ng/ml (N – 3.2 ng/ml). The alterations were marked in the concentracion of gonadotrops as well. The concentration of LH on the 16th day: made 1.4 ng/ml, and on the 45th day decreased to 1.2 ng/ml (N – 2.6 ng/ml), FSH  on the 16th day made 2.6 ng/ml, and by the 45th day decreased to 2.2 ng/ml (N – 2.4 ng/ml).

Thus, intoxication with phenol vapor causes definite alterations in the organisms of male rats, which are expressed in the quantitative alterations of gonadotrope and sex hormones. The deeper and more detailed study of this question is the target of our next research.

 

 

KVIRIKASHVILI M.K., DATUNASHVILI I.V., METREVELI D.S.

CORRECTION OF OXIDATIVE METABOLISM IN RAT LIVER THYROTOXICOSIS

BY USING OLIGOKRINE

Experimental and Clinical Medicine, 2006, 2, pp.14-17.

 

Tbilisi State Medical Academy;

Tbilisi State Medical University, Georgia

 

The present study aimed to evaluate disturbance of oxidative metabolism in liver and protective   efficacy of oligocryne thyreotoxicosis. It was shown that under the effect of L-thyroxine, intensification of nitric oxide synthesis and activation of lipid peroxidation processes in liver are  observed. These changes become evident via the accumulation of free radical species of nitric oxide and lipid peroxides, mitochondrial electron transport chain impairment at NADH ubiquinone-reductase site and the accumulation of ubisemiquinones. Administration of oligokrine leads to the relative normalization of electron transport in the mitochondrial respiratory chain and to the decrease in NO level in liver EPR specter. These results, as we suggest, are due to antioxidant effect of this preparation which inhibits the intensification of oxidative stress and hyperexpression of iNOS in liver tissue. By the suppression of excess NO synthesis oligocrine exerts positive effect on body thyroid status and prevents an excessive activation of oxidative processes in experimental animals.

 

 

ZHORZHOLADZE E., KHUGASHVILI R., SANIKIDZE T., JIKIA I.

ANTIPHOSPHOLIPID ANTIBODIES IN PREECLAMPSIA

Experimental and Clinical Medicine, 2006, 2, pp.18-21.

 

Tbilisi State Medical University, Georgia

 

The aim of the study was to establish of correlations between the availability of antiphospholipid antibodies and dynamics of  oxidative homeostasis parameters and nitric oxide content in blood of pregnant women with signs of preeclampsia (gestation 25-36 weeks).

43 women were investigated. The general group consisted of 28 pregnant women with signs of preeclampsia (systolic arterial pressure > 140 mm Hg, diastolic pressure  > 90 mm Hg, proteinuria and edema) and antiphospholipid antibody presence in blood. The control group consisted of 15 healty pregnant women.

A positive correlation was revealed  between the availability of antiphospholipid antibodies, intensification of oxidative stress, and development of endothelial dysfunction in the organism of pregnant women. Conclusion: in pregnant women there exists a cause-consequence dependence between antyphospholipid antibodies formation, oxidative stress and preeclampsia syndrome development.

 

 

BERIDZE L., KATSITADZE A., SANIKIDZE T., KATSITADZE T.

CHANGES IN THE REDOX-SYSTEM OF THE ORGANISM DURING ROSACEA

Experimental and Clinical Medicine, 2006, 2, pp.21-23.

 

TSMU Chair of Dermatology and Venerology, Tbilisi, Georgia

 

Indexes of the blood Redox-system were studied in light (20 patients) and severe (20 patients) forms of Rosacea. On the basis of the analysis of the results an intensification of oxidative stress takes place in the organism and is expressed as inactivation of the antioxidant system of blood, emergence of Fe2+ and Mn2+ions, superoxide- and peroxideradicals and development of methgemoglobinemia. Contraction of the contents of nitrogen oxide in blood is the result of transformation to cytotoxic peroxinitrite and of nitrosilation of hemoglobin. It should be pointed out that surplus formation of nitro-hemoglobin (HbNO) and peroxinitrite under Rosacea is a secondary pathogenesis link, which advances aggravation of the disease.

 

 

ABZHANDADZE T., KVEZERELI-KOPADZE A., JAPARIDZE E., TKHILAVA N.

MECHANISMS OF REDOX-HOMEOSTASIS DISORDERS DURING HYPERBILIRUBINEMIA

Experimental and Clinical Medicine, 2006, 2, pp.23-26.

 

I.Pagava Scientific Research Institute of Pediatry, Tbilisi, Georgia

Tbilisi State Medical University, Georgia

 

The aim of the study was to investigate free radical oxidation intensity in blood of newborns with hyperbilirubinemia. The results of the study testify that intensification of lipoperoxidation in blood of newborns with hyperbilirubinemia promotes disturbance of the erythrocyte membrane, decrease in of their resistance and deformability, intensification of hemolysis and accumulation of methemoglobine. Correspondingly decreases erythrocyte’s ability to connect hemoglobin, decreases the oxyhemoglobin content in blood. It was concluded, that intensification of oxidative stress during hyperbilirubinemia is conditioned by presence of additional bilirubin metabolism dependent agent (hem oxygenase), which promotes release of redox-active iron from the haem and intensification of oxidative stress.

 

 

KAPANADZE N., CHICHINADZE N., SUMBADZE TS.

ENDOCRINE FUNCTION OF HEART DURING VIBRATION STRESS IN EXSPERIMENT

Experimental and Clinical Medicine, 2006, 2, pp.26-28.

 

AL.Natishvili Institute of Experimental Morphology Georgian AS, Tbilisi, Georgia

 

The target of our research is reaction of the auricular-atrial-natriuretic factor on vibration pathology. The research was held on three groups of 70 grown-up male rats (including 10 intact) each weighing 180-200g. The vibroséance continued for 1 hour daily during 2 months. For the purpose of correction of such alternations that developed in the organism during vibration pathology, hypodermic injections of liquid oxygen (1drop is dissolved in 1 ml of distilled water) in the dose of 0.1 ml were additionally made to the 2nd group every other day. To the 3rd group, after the vibroséance 0.01 ml of Retabolil was additionally injected once a week. After the experiment, on the 20th, 40th and 60th days we carried out decapitation of the animals in conditions of general anesthesia. In the blood plasma we defined the concentration of auricular atrial natriuretic factor (ANF) by means of the radioimmunology analysis method. The received data were processed by the variation statistics method. All initial values were taken as 100%. The research showed that the concentration of ANF in the 1st group of animals reached 265% for the 20th day, of the 40th day the ANF concentration fluctuated within the limits of 225%, and on the 60th day its concentration decreased to 193%. For the 20th day, in the 2nd group the content of ANF in the blood plasma decreased to 96%, for the 40th day fluctuated within the limits of 85%, and on the 60th day its concentration decreased to 63%. In the 3rd group of animals for the 20th day ANF concentration reached 357%, for the 40th day fluctuated within the limits of 317%, and on the 60th day its concentration decreased to 234%. Reasoning from the received data, the reactions developed in the 1st, 2nd and 3rd groups are different. By the data received in the 1st group we may assume the organism’s compensative mechanism. In the 2nd group, in accordance with the existing data the economical expenditure of energy and oxygen took place. We can assume, according to the alternations in the 3rd group, that Retabolil as anabolic steroid intensified the albumen synthesis and the received reaction can be caused as a result of albumen synthesis intensification.

 

 

PETRIASHVILI SH., GABUNIA L., VARDOSANIDZE T., TSERTSVADZE L.

CLINICAL SIGNIFICANCE OF ß-ADRENOBLOCKERS - NEBIVOLOL  AND

CARVEDILOL - IN THE THERAPY AFTER MYOCARDIAL INFARCTION

Experimental and Clinical Medicine, 2006, 2, pp.28-32.

 

Tbilisi state Medical University, Tbilisi, Georgia

 

The effectiveness of ß-adrenoblockers in the therapy of myocardial infarctions is confirmed by a lots of experimental and clinical investigations. Though the effectiveness of the III generation of ß-blockers (with additional vasodilatative features) is yet unclear.

The aim of the study was a comparative analysis of Nebivolol and Carvedilol influence on clinical-haemodinamic characteristics of patients once carried myocardial infarction.

An open comparative investigation was carried out on 78 man (aged 35 to 65). The patients were randomized in two groups: Nebivolol (5,0 mg/kg) was administered to I group (39 patients) during three months. Carvedilol (25,0 mg/kg) was administered to the II group (39 patients). The level of coronary and myocardial insufficiency and effectiveness of the therapy was determined by clinical-instrumental investigation (tredmil-test, echocardiography). The characteristics were estimated before and after 3-month therapy.

The influence of carvedilol was better than nebivolol in total amount of unreliable cardio-vascular outcome in patients who only once underwent myocardial infarction. Antiarrhythmic action and side effects quantity of carvedilol and nebivolol were the same in both groups.

 

 

 

DIDEBULIDZE N..KAKABADZE M.

CHANGE OF THE CONTENT OF RECEPTORS TO SEX STEROIDS IN THE CARDIAC MUSCLE

OF MALE-RATS DURING STIMULATION OF THE MYOCARDIUM BY ADRENALINE AND PITUITRIN

Experimental and Clinical Medicine, 2006, 2, pp.32-33.

 

A.Natishvili Institute of Experimental Morphology, Georgian AS, Tbilisi, Georgia

 

A radiometric study of the myocardium has revealed receptors to sex steroids in the atria, auricles and ventricles. Stimulation of the myocardium by pituitrin and adrenaline incriased the quantity of androgene and estrogen receptors in all the studied sites of the heart.

 

 

GUDUSHAURI N., JANGAVADZE M.,  QIRAKISHVILI Z., GORGOSHIDZE G.

MORPHO-FUNCTIONAL CHANGES OF THE PLACENTA IN PREGNANT WOMEN WITH  PYELONEPHRITIS

Experimental and Clinical Medicine, 2006, 2, pp.33-36.

 

A.Natishvili Institute of Experimental Morphology, Georgian Academy of Sciences,Tbilisi

I.Phagava Research Institute of Pediatrics, Tbilisi, Georgia

 

 

Morphological features of the placenta in pregnant women with chronic pyelonephritis have been studied. There was revealed a considerable amount of sclerotic villi and necrosis reflecting compensatory – adaptive processes.

A morphometric  study of the afterbirths showed that placenta affected with pyelonephritis has large area of the intervillous space and small area of chorionic villi involved in gas exchange,  nutrient supply and other components to the fetus.

 

 

 

BEJANIDZE Z., IAKOBIDZE B., SHAVDIA N.

THE USE OF VITEX IN VITILOGOTREATMENT

Experimental and Clinical Medicine, 2006, 2, pp.36-38.

 

Tbilisi Research Institute of Dermatology and Venerology, Georgia

 

Vitiligo is one of the actual problems in Dermatology. Our attention has been attracted by the preparation “Vitix”. In dermatological practice it  is used as a gel.

We have examined 12 patients (8 females and 4 males). The observation was held for 6 months. Clinical improvement was assessed in percents. The following reactions were noted: Recovery, improvement, no improvement. As a result of the research we found the improvement of a condition in 83/3% (10) patients, no improvement was found in 16.7% (2) patients. Thus, the efficacy of the preparation “Vitix” is obvious, as in 83.3% patients was marked the improvement of the condition from insignificant to average.

Due to the preparation “Vitix” the regeneration of the physiological balance of free radicals takes place in epidermal cells (melanocytes and ceratinocytes). The preparation is well tolerated. In the future “Vitix” will become one of the main preparations for vitiligo treatment.

 

 

 

 

SMIRNOV I., SOROKINA T., GORIUNOV A., NADIRASHVILI S., MAIKIBAEVA G.

NITRIC OXIDE DURING CHRONIC LUNG DISEASES IN CHILDREN

Experimental and Clinical Medicine, 2006, 2, pp.38-40.

 

Scientific Center of Pediatry of RAMS, Moscow, Russia

A.Natishvili Institute of Experimental Morphology, Georgian Academy of Sciences,Tbilisi

 

Thus, an increased local production of nitric oxide during chronic inflammatory lung diseases is an important component of antipathogenic protection which is put into effect via the cascade of interactions in the pathogen-immunocyte-macrophage system, and is accompanied by the increased production of such bacteriostatic agents as NO peroxide compounds, affectory cells. These compounds, in their turn, stimulate a further increase in nitric oxide endogenic production that executes a regulatory role in the local cellular reaction on the pathogen.

 

 

PACHKORIA V., KARAZANASHVILI G., BURKADZE G.

THE RESULTS OF IPSS QUESTIONNAIRE AND QUALITY OF LIFE IN THE PATIENTS WITH

LOCALIZED AND NON-LOCALIZED FORMS OF PROSTATE CANCER

Experimental and Clinical Medicine, 2006, 2, pp.40-43.

 

Departments of Topographic Anatomy, Urology, and Pathanatomy of TSMU, Tbilisi, Georgia

 

 

We have investigated 144 males with prostate cancer after radical prostatectomy. According to the postoperative material of pathomorphological researches, the patients were devided into two groups: 1) 68 patients with prostate cancer of localized form; 2) 76 patients with non-localized forms. According to IPSS questionnaire, the quality of clinical reflection of disease was moderate in prostate cancer of localized forms and severe – during non-localized forms. The evaluation of life quality showed that 33.8% of patients with prostate cancer evaluated their quality of life as satisfactory, 25% mentioned mixed feelings, 23. % indicated unsatisfactory condition, and 17.6% had complaints. The patients with non-localized cancer showed mixed feeling (27.6%), 23.7% evaluated their condition as unsatisfactory, 25% had complaints, and 23.7% had depression.

 

 

KIPSHIDZE N., TALAKVADZE T., BREGVADZE N., MAMATSASHVILI I., TABAGARI S.

CLINICAL LABORATORY INDEXES AND THEIR ANALYSIS BY ETIOLOGY AND

NYHA CLASS IN PATIENTS WITH CHRONIC HEART FAILURE

Experimental and Clinical Medicine, 2006, 2, pp.44-49.

 

National Center of Therapy;

Tbilisi State Medical University, Tbilisi, Georgia

 

Aims. Heart failure is usually associated with impaired systolic ventricular function but may also present with preserved systolic ventricular function . The development and progression of heart failure depends on a variety of systemic, cellular, and molecular abnormalities. This study therefore examined clinic laboratory indexes and analyzed them by syndrome etiology and gravity.

Methods and Results. 102 patients with chronic heart failure of mixed etiology were examined. BMI, IS, EF%, Bilirubin, creatinine, total protein, albumin, iron, glucose, ferritin, TC, TG, HDL-C, LDL-C, VLDL-C, IA, TC/HDL, log(TG/HDL-C), Apo-B, Apo-A were investigated. No difference in any parameters was observed between the ishemic and non ishemic etiology groups. In NYHA class subgroups significant differences were in EF %, IS, creatinine clearance, albumin, ferritin, TG, log(TG/HDL-C) and Apo-B.

Conclusion. Thus, the progression of Heart failure is associated not only with changes in LV loading conditions, but also with abnormalities of biochemical tests analysis of which is necessary for more effective treatment.

 

 

MAKHARASHVILI P., SANIKIDZE T., MAMAMTAVRISHVILI I., KINTRAIA N.

THE ROLE OF NITRIC OXIDE IN THE MECHANISM OF FETOPLACENTAL CIRCULATION

DISORDER DURING PATHOLOGICAL COURSE OF PREGNANCY

Experimental and Clinical Medicine, 2006, 2, pp.49-52.

 

Chachava Research Institute of Perinatal Medicine, Obstetrics and Gynecology;

Tbilisi State Medical University, Tbilisi, Georgia

 

In the development of pathological pregnancy, a great attention is paid to the lesion of oxidizing metabolism and functional-structural changes in the placenta, as well as to the lesion of fetoplacental circulation. There exist data that in fetoplacental circulation lesion an important role belongs to the endothelial dysfunction in which nitric oxide metabolism has not a little importance. The goal of the research: Determination of the correlation between the nitric oxide content in the umbilical blood/placenta and pregnancy course. In the umbilical blood of the parturients with preeclampsia syndrome, a high content of these complexes was revealed. The existence of these complexes in low concentrations in the blood during physiological pregnancy points to their important role in pregnancy development. The formation of these complexes is conditioned by the decrease in parcial pressure of oxygen at the beginning of delivery, by hypoxia and insufficient irrigation of the placenta with blood during preeclampsia. As a result of depositing in the nitrozile hemocontainer complexes (HbNO), NO loses activity which, in its turn, further aggravates placenta dysfunction. It possible to assume that these complexes play an important role in the mechanism of the oxygen-dependent regulation of the relaxing NO activity during delivery.

 

 

MOSEMGVDLISHVILI N.G., CHICHINADZE N.I., BAKURADZE L.Z.

DEPENDENCE OF ERYTHROCYTES FUNCTIONS ON THE SEVERITY OF PERIODONTAL DISEASE

Experimental and Clinical Medicine, 2006, 2, pp.53-55.

 

A.Natishvili Institute of Experimental Morphology, Georgian AS, Tbilisi, Georgia

 

 

Periodontal disease is one of the most widespread diseases, but the questions of its pathogenesis are not clear. The aim of the study was to reveale the dependency of deformability, resistance and oxygen transport functions of erythrocytes on the intensity of oxidative stress during periodontal disease. The results of the study show that the intensity of the lipid peroxidation correlates with disease severity. During grave form of the disease the physical (deformability, resistance) and physiological (oxygen transport) properties of erythrocytes decrease in correlation with the intensification of free radical oxidation.

 

 

SIKHARULIDZE M., NIORADZE I., TKHILAVA N.,  SANIKIDZE T., CHKHIKVISHVILI I.

INFLUENCE OF CITRUSES EXTRACT ON LIVER METABOLISM DURING OBESITY

Experimental and Clinical Medicine, 2006, 2, pp.55-59.

 

Institute of Medical Biotechnology of Georgian Academy of Sciences, Tbilisi, Georgia

 

Obesity and dislipidemia often cause heart disease, metabolic syndrome and steatohepatites development. The aim of our study was to investigate of liver metabolism during obesity and its correction with citruses extract.

On the basis of the obtained data we can conclude that during high caloried diet the excess of free fatty acids promotes decreasing activity of mitochondrial electron transport chain and oxidative phosforilation, decreasing  intensity of Limon acid cycle, transamination of -ketoglutarate, oxidation of free fatty acids and intensification of oxidative stress in hepatocytes. Abundant accumulation of triacilgliceroles causes development of fatty infiltration of hepatocytes. The influence of citrus preparations restores  mitochondria electron transport chain in hepatocytes, promotes decreasing of peroxidation processes intensity, elevation of Limon cycle and -ketoglutarate transamination intensity, improving ALT and AST concentration in blood. During influence of a citrus preparation the mass animals body, epididimal fat mass and liver mass don’t change as compared to the control level.  

 

 

SHAVDIA M., GVAMICHAVA R., ABESADZE J., AROSHIDZE T., KACHAKHIDZE G., METIVISHVILI G., SHAVDIA N.

SYMPTOM PREVALENCE IN PATIENTS WITH ADVANCED CANCER

 Experimental and Clinical Medicine, 2006, 2, pp.60-64

 

Tbilisi State Medical University, Palliative Care Clinic of Cancer Prevention Center, Georgia

 

In 2003, 422 cases of cancer of various organs were studied in Cancer National Center (Georgia) in order to determine the most frequent symptoms at the stage of incurability.  All cases were selected at random. The diagnoses were confirmed by morphology test. Health status of the patients were assessed by score gradation of functional health status (ECOG-WHO-score performance status) recommended by WHO. Symptoms were recorded on the basis of disease history.

The study findings showed that: 1. Only 2.1 % of incurable cancer (ECOG-WHO score-I) patients seek medical care when they experience initial symptoms of the disease, and 92.9 % of the patients apply to medical care when they present advanced complaints (ECOG-WHO score-II-IV), i.e.after they are partially of fully confined to bed  because of the worsened health status; 2. The most frequent symptoms for determining cancer incurability (ECOG-WHO score-I-IV) are: asthenia (82.0%), pain (75.6%), depression (33.4%), anorexia (27.0%), anemia (22,0%), constipation (21.6%), difficulties in breathing (20.1%), nausea/vomiting (14,9%) and weight loss (54.7%), limitation in dayly activities (moving) (17.1%), ascitis (12.8%), carcinomatosis (8.1%), tumoral pleuritis (8.8%). In addition, frequency of symptoms was increasing in parallel with advancing of ECOG-WHO-performance status; 3. Delayed refferal to specialized medical care facility must be associated with low public awareness or financial matters. That is one of the contributing factors to develop cancer incurability. All the above mentioned should be considered in   palliative care providing.

 

 

 

BITSKINASHVILI M., KIPSHIDZE N., KAKURIDZE N., KHAKHUTAISHVILI I., NIKOLAISHVILI G.

THE LEVELS OF C- REACTIVE PROTEIN AND FIBRINOGEN IN OBESED PATIENTS

WITH CORONARY HEART DISEASE

Experimental and Clinical Medicine, 2006, 2, pp.64-67.

 

National Center of Therapy, Tbilisi, Georgia

 

The aim of the study is to determine relationship between C- reactive protein, fibrinogen levels and body fat composition in patients with coronary heart disease and without it.

Materials and Methods. We investigated 48 men whom were devided into four groups. I- group  12 obesed subjects with coronary disease(CHD), II-group - 12 non-obese  patients with CHD, III- control group  - 12 obesed men , IV-control group - 12 non-obese subjects .

There was revealed a positive correlation between  the levels of C-reactive protein, fibrinogen, body fat composition  and indices of blood pressure in obesed patients.

Conclusions. 1.Obesity is correlated with levels of C-reactive protein in condition of CHD and without it, but relationship between  fibrinogen  levels and obesity  were revealed only in patients with CHD.

2.The indices of artherial blood pressure are related with body fat content.

3.The risk of dyslipidemia is connected with high body fat composition in patients with CHD and without it.

 

 

KATAMADZE N., ZHORZHOLIANI L., CHKHARTISHVILI E.

INDOOR ECOLOGY AND BRONCHIAL ASTHMA IN CHILDREN

Experimental and Clinical Medicine, 2006, 2, pp.67-70.

 

I.Pagava Scientific-Research  Institute of Pediatrics, Tbilisi, Georgia

 

The influence of different characteristic features  of indoor environment on bronchial asthma occurrences in children population of Batumi has been studied. Under the observation were 2110 children (age - 1 to 15 years). Indoor risk factors were studied by the interviewing method. To evalua risk factors the “case-control” method was used. Statistical analysis was  carried out  on the basis of SPSS v.12 program.

According to the obtained data indoor ecological factors (dust collectors, humidity, mould, active cigarette smoking, house heating and number of family members) exer a significant influence on of sensibility occurrence the frequency of house dust mite, mould fungi and other everyday allergens and bronchial asthma formation in children.

 

 

 

 

 

CHACHIA G., GIORGOBIANI M.

ANTIOXIDANTS IN COMPLEX TREATMENT OF ENCEPHALOPATHY

Experimental and Clinical Medicine, 2006, 2, pp.70-72.

 

Pediatric Clinic of TSMU, Tbilisi, Georgia

 

An especially important place belongs to the viral toxic and metabolic nature of the etiology of infectious-dystrophic encephalopathy. At the beginning these changes have functional character; afterwards, as a result of influences of toxic substances, organic infringements and the infringements of the electrolytic balance develop. Getting through hemato-encephalitic barrier free radicals cause a circuit of infringements which can be distributed in the following way: infringement of brain blood circulation of lipid metabolism, glucids and fibers, electrolyte periventricular leukomalation, intraventricular hemorrhages. Later develop porencephalic cysts, hydrocephaly of normal, hydro- and hypertensive type. In the article a great attention is paid to the important role of complex treatment (surgical, early antioxidative and metabolic) of sick children in the early postnatal period. The article summarizes the analysis of 56 diseased children who were successfully treated with the dry extract of grape seed – “Antiox G” containing 68-90% active flavonoids (17% catechines, 38% proanthocyanidins).

 

 

CHOCHUA1 S., SUJASHVILI3 L., PHANTSULAIA1,3 I., JGENTI1 M., CHIKOVANI1,2 T.

THE EFFECT OF PLAFERON LB ON LIPOPOLYSACCHARIDE (LPS)-INDUCED LETHALITY

IN EXPERIMENTAL MODEL OF ENDOTOXEMIA

Experimental and Clinical Medicine, 2006, 2, pp.72-75.

 

Institute of Biotechnology, Georgian AS1

TSMU2; State Medical Academy3, Tbilisi, Georgia

 

Activation of the immunoinflammatory system during gram-negative bacterial infections is a common cause of septic shock, a life-threatening condition characterized by functional derangements in many organs. Gram-negative bacteria provoke septic shock by releasing their cell wall component lipopolysaccharide (LPS) into the circulation.

It is generally accepted that type 1 cytokines play a pivotal role in the pathogenesis of endotoxic shock conditions through their proinflammatory and vasoactive properties. The balance between the type 1 and type 2 cytokines may influence the host response to endotoxaemia. Therefore, pharmacological compounds inhibiting the production of type 1 cytokines as well as up-regulating the expression of type 2 cytokines may be suitable candidates for the prevention/treatment of endotoxaemia. Plaferon LB is a compound with powerful antiinflammatory and immunomodulatory effects. Thus, the main aim of our study was to evaluate the effects of Plaferon LB on the experimental lethal endotoxaemia in mice.

Prophylactic treatment with Plaferon LB and anti-TNF-alpha polyclonal antibody significantly diminished the lethality of the mice (from 83,3% to 25% and 33,3% respectively). “Therapeutically administered” Plaferon LB also diminished LPS-induced lethality (33,3%). However, administering anti-TNF-alpha Ab failed to counteract the lethal effects (75%).

In conclusion, Plaferon LB successfully counteracted LPS-induced lethality in mice regardless of whether it was given prior to or early after endotoxin challenge.